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Purified anti-STAT5 Phospho (Tyr694) Antibody

Pricing & Availability
Clone
A17016B.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 5A
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_A17016B-Rec_PURE_STAT5-P_Antibody_031720
Human peripheral blood mononuclear cells were treated with (filled histogram), or without (open histogram) recombinant human IL-2 for 15 minutes, fixed with fixation buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with purified anti-STAT5 Phospho (Tyr694) (clone A17016B.Rec), and followed by anti-mouse IgG1, κ PE. Data was gated on the lymphocyte population.
  • 1_A17016B-Rec_PURE_STAT5-P_Antibody_031720
    Human peripheral blood mononuclear cells were treated with (filled histogram), or without (open histogram) recombinant human IL-2 for 15 minutes, fixed with fixation buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401) then stained with purified anti-STAT5 Phospho (Tyr694) (clone A17016B.Rec), and followed by anti-mouse IgG1, κ PE. Data was gated on the lymphocyte population.
  • 2_A17016B-Rec_PURE_STA5_Phospho_Antibody_2_041420.png
    Whole cell extracts (15 µg protein) from serum-starved NK-92 cells untreated (-) or treated (+) with 100 ng/mL IL-2 for 20 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of purified anti-STAT5 Phospho (Tyr694) antibody (clone A17016B.Rec) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal STAT5 loading was confirmed by probing membranes with 1.0 µg/mL (1:500 dilution) of purified anti-STAT5 antibody (clone 9C8B50, Cat. No. 660202). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker
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936902 100 µg £182
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Description

STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant. Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Peptide from Human Stat5A phosphorylated at Tyr694
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This recombinant antibody clone is derived from clone A17016B. No changes or modifications were done to the antibody sequence or scaffold.

Product Citations
  1. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
RRID
AB_2861121 (BioLegend Cat. No. 936902)

Antigen Details

Structure
794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
Distribution

Cytoplasm; phosphorylated form translocates to the nucleus.

Function
STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Antigen References
  1. Bell BD, et al. 2013. Nat. Immunol. 14:364.
  2. Cholez E, et al. 2012. Leukemia. 26:2390.
  3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146.
  4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18.
  5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168.
  6. Xu J, et al. 2009. J. Neurosci. 29:2022.
Gene ID
6776 View all products for this Gene ID
UniProt
View information about STAT5 Phospho Tyr694 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 03/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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