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Purified anti-TSG101 Antibody

Pricing & Availability
Clone
W19019A (See other available formats)
Regulatory Status
RUO
Other Names
Tumor Susceptibility Gene 101, Tumor Susceptibility Gene 101 Protein, ESCRT-I Complex Subunit TSG101, VPS23 , TSG10
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19019A_PURE_TSG101_Antibody_1_080921.png
Whole cell extracts (15 µg total protein) from SH-SY5Y, A549, Daudi, and HeLa (positive control) lysates, and NIH/3T3 lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-TSG101 antibody (clone W19019A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19019A_PURE_TSG101_Antibody_1_080921.png
    Whole cell extracts (15 µg total protein) from SH-SY5Y, A549, Daudi, and HeLa (positive control) lysates, and NIH/3T3 lysates were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-TSG101 antibody (clone W19019A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19019A_PURE_TSG101_Antibody_2_080921.png
    Whole cell extracts (15 µg total protein) from HeLa cells treated with non-targeting control siRNA (siCON) or siRNA targeting TSG101 (siTSG101) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-TSG101 antibody (clone W19019A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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934301 25 µg £81
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934302 100 µg £201
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Description

Tumor Susceptibility Gene 101, TSG101, is a member of the ubiquitin E2 variant (UEV) subfamily of ubiquitin-conjugating enzymes. It is vital to transcriptional regulation, cytokinesis, tissue homeostasis, cell cycle regulation, and cell growth, differentiation, and proliferation. TSG101 is an essential component of the ESCRT-1 (endosomal sorting complexes required for transport) complex, which functions in multivesicular body biogenesis, endosomal trafficking, management of damaged proteins, recycling of membrane receptors, and cellular abscission. Within ESCRT-1, TSG101 binds to ubiquitinated proteins, aids in the formation of multivesicular bodies (MVBs), and recruits subsequent ESCRT components to the complex. Its role as part of ESCRT also makes TSG101 vital to cellular signal transduction, the recycling of receptor tyrosine kinases (RTKs), and Src-mediated migration. TSG101 is essential to the pathogenesis of several viruses - including HIV, influenza, and human papillomavirus - through its facilitation of interactions between the ESCRT complex and viral matrix proteins via the P(T/S)AP motif, as well as the budding and egress of enveloped viruses. Once predicted to behave as a tumor suppressor due to its role as a negative growth regulator, TSG101’s overexpression in colon, thyroid, breast, ovarian, and lung cancers suggests that TSG101 may actually function as an oncoprotein and play a role in cancer progression. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant TSG101 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
WB- KO/KD - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ICC testing was conducted on HeLa siRNA control cells (positive control) and HeLa TSG101 siRNA knockdown cells (negative control) using three different fix/perm methods: MeOH fixation, PFA + Triton X-100, and PFA + MeOH. Staining was observed but there was no decrease in signal observed in the siRNA knockdown cells, indicating staining was not specific for TSG101. We therefore do not recommend this clone for the application. 

RRID
AB_2894522 (BioLegend Cat. No. 934301)
AB_2894522 (BioLegend Cat. No. 934302)

Antigen Details

Structure
TSG101 is a 390 amino acid protein with a predicted molecular weight of 43 kD.
Distribution

Ubiquitously expressed/ nucleus, endosome, cytoplasm, endosome, and cytoskeleton.

Function
Cell cycle, Growth regulation, Host-virus interaction, transcription
Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References

1. Ferraiuolo R, et al. 2020. Cancers. 12:450.
2. Kumar B et al. 2016. PLOS Pathogens. 12:e1005960.
3. Qadir M and Zafar M. 2017. Crit. Rev. Eukaryot. Gene Expr. 27:237.
4. Tu C, et al. 2011. BioArchitecture. 274:6056.
5. Wagner K, et al. 2003. Mol. Cell. Biol. 1:45.

 

Gene ID
7251 View all products for this Gene ID
UniProt
View information about TSG101 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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