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Purified anti-V5 Epitope Tag Antibody (Previously Covance catalog# PRB-189P)

Pricing & Availability
Clone
Poly29038 (See other available formats)
Regulatory Status
RUO
Other Names
GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, Rev, V5 epitope tag
Previously
Covance Catalog# PRB-189P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
A_Poly29038_PURE_V5_Epitope_Tag_Antibody_WB_112817
Western blot analysis of cell lysates from HeLa cells transfected without or with V5-tagged plasmid using V5 Rabbit primary antibody (clone Poly 29038, 1:10000 dilution, 0.1 µg/ml) and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807, 1:8000 dilution). was used as a loading control.
  • A_Poly29038_PURE_V5_Epitope_Tag_Antibody_WB_112817
    Western blot analysis of cell lysates from HeLa cells transfected without or with V5-tagged plasmid using V5 Rabbit primary antibody (clone Poly 29038, 1:10000 dilution, 0.1 µg/ml) and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807, 1:8000 dilution). was used as a loading control.
  • B_Poly29038_PURE_V5_Epitope_Tag_Antibody_ICC_092523
    CHO cells untransfected (negative control, panel A) and transfected (positive control, panel B) with a multi-tag construct containing the V5 epitope were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes. The cells were then blocked with 5% FBS for 1 hour at room temperature. Cells were stained with 2 µg/mL of purified anti-V5 Epitope Tag (clone Poly29038), followed by incubation with Alexa Fluor® 594 goat anti-rabbit IgG (Cat. No. 406418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 µm
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903802 25 µL £61
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903801 100 µL £227
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Description

The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against V5 epitope tag recognize tagged proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal V5 antisera was raised against a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian parainfluenza virus 5 conjugated to KLH.
Formulation
Phosphate-buffered solution with 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC, IHC, ELISA - Verified
Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 0.2 µg/mL (1:5000 - 1:10000 dilution). For immunocytochemistry, a concentration range of 0.1 - 5.0 μg/mL is recommended. For immunohistochemistry, a dilution range of 1:100 - 400 is suggested.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry, and ELISA.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

The antisera was purified via affinity chromatography using the peptide immobilized on solid support.

This product may contain other non-IgG subtypes.

Product detects V5 epitope tag in ICC using Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol, or with fixation/permeabilization with 100% ice-cold methanol.

Application References

(PubMed link indicates BioLegend citation)
  1. J Dixelius, et al. 2003. J. Biol. Chem. 278:40973.
Product Citations
  1. Sluder AE, et al. 2022. Front Immunol. 13:901372. PubMed
  2. Xu W, et al. 2020. Immunohorizons. 0.631944444. PubMed
  3. Cui Y, et al. 2020. J Allergy Clin Immunol. 391:145. PubMed
RRID
AB_2565034 (BioLegend Cat. No. 903802)
AB_2565034 (BioLegend Cat. No. 903801)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about V5 Epitope Tag on UniProt.org

Other Formats

View All V5 Epitope Tag Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-V5 Epitope Tag Poly29038 WB,ICC,IHC,ELISA,Purification
Go To Top Version: 8    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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