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Purified anti-V5-tag Antibody

Pricing & Availability
Clone
7/4 (See other available formats)
Regulatory Status
RUO
Other Names
GKPIPNPLLGLDST epitope tag
Isotype
Mouse IgG2b, κ
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Product Citations
publications
7slash4_PURE_V5_Antibody_1_WB_093015
Different amounts of V5 tagged proteins (Genescript, M0101) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal V5-tag (clone 7/4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 7slash4_PURE_V5_Antibody_1_WB_093015
    Different amounts of V5 tagged proteins (Genescript, M0101) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal V5-tag (clone 7/4) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 7slash4_PURE_V5_Antibody_2_IP_093015
    200ng V5 tagged protein was resuspended in PBS. Lane 1 was immunoprecipitated with 2 µg mouse IgG. Lane 2 was immunoprecipitated with 2 µg anti-V5-tag (clone 7/4) antibody. Lane 3 was immunoprecipitated with 2 µg anti-V5-tag (clone poly290238) antibody. Lane 4 was loaded with 50ng V5 tagged protein directly as a positive control. Western blot analysis was performed using mouse anti-V5 tag antibody (clone 7/4) and followed by HRP-anti-mouse IgG.
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680601 25 µg £70
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680602 100 µg £182
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Description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunoprecipitation, the suggested use of this reagent is 1-5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Bussey KA, et al. 2018. PLoS Pathog. 11:43. PubMed
  2. Hertzog J, et al. 2022. EMBO J. :e109217. PubMed
  3. Pham TH, et al. 2020. Cancer Discov. . PubMed
  4. Lou Y, et al. 2020. Cell Mol Immunol. 1.439583333. PubMed
  5. Stempel M, et al. 2019. EMBO J. 38:. PubMed
RRID
AB_2566386 (BioLegend Cat. No. 680601)
AB_2566386 (BioLegend Cat. No. 680602)

Antigen Details

Structure
14 amino acid peptide (GKPIPNPLLGLDST)
Function
The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
Biology Area
Cell Biology
Antigen References

1. Uhlen M, et al. 1990. Meth Enzymol. 185:129.
2. Hanke T, et al. 1995. J. Virol. Methods 53:149.
3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551.
4. Thomas SM, et al. 1988. Cell 54:891.

Gene ID
NA
UniProt
View information about V5-tag on UniProt.org
Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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