Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) Antibody, Ly-6G, 1A8

Pricing & Availability

Clone: 1A8 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ
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285146

50 µg

£230

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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Ly-6G transfected EL-4J cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Antigen Details

Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: NA
UniProt: View information about Ly-6G on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 594 anti-mouse Ly-6G	1A8	IHC-F,SB
Purified anti-mouse Ly-6G	1A8	FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G	1A8	FC,IHC
FITC anti-mouse Ly-6G	1A8	FC
PE anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 647 anti-mouse Ly-6G	1A8	FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G	1A8	FC
APC anti-mouse Ly-6G	1A8	FC
PerCP/Cyanine5.5 anti-mouse Ly-6G	1A8	FC
PE/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 700 anti-mouse Ly-6G	1A8	FC,IHC
APC/Cyanine7 anti-mouse Ly-6G	1A8	FC
Alexa Fluor® 488 anti-mouse Ly-6G	1A8	FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G	1A8	FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G	1A8	FC
Ultra-LEAF™ Purified anti-mouse Ly-6G	1A8	FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G	1A8	FC
Purified anti-mouse Ly-6G (Maxpar® Ready)	1A8	FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 711™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 605™ anti-mouse Ly-6G	1A8	FC
Brilliant Violet 785™ anti-mouse Ly-6G	1A8	FC
PE/Dazzle™ 594 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 750 anti-mouse Ly-6G	1A8	FC
PerCP anti-mouse Ly-6G	1A8	FC
TotalSeq™-A0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-C0015 anti-mouse Ly-6G	1A8	PG
TotalSeq™-B0015 anti-mouse Ly-6G	1A8	PG
Spark Blue™ 550 anti-mouse Ly-6G	1A8	FC
Spark NIR™ 685 anti-mouse Ly-6G	1A8	FC
Spark YG™ 593 anti-mouse Ly-6G	1A8	FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
PE/Cyanine5 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody	1A8	FC
Spark UV™ 387 anti-mouse Ly-6G	1A8	FC
PE/Fire™ 640 anti-mouse Ly-6G	1A8	FC
Spark YG™ 570 anti-mouse Ly-6G	1A8	IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™)	1A8	FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™)	1A8	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/05/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.