Spark Red™ 718 anti-human CD103 (Integrin αE) (Flexi-Fluor™) Antibody, CD103, Ber-ACT8

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Clone: Ber-ACT8 (See other available formats)
Regulatory Status: RUO
Workshop: V A067
Other Names: Integrin alpha E (ITGAE)
Isotype: Mouse IgG1, κ
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Product Citations: publications

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285063

50 µg

£257

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Description

CD103 is a type I transmembrane glycoprotein also known as αE integrin, integrin αIEL chain, and human mucosal lymphocyte antigen 1. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. Treg cells express high level of CD103. Hairy cell leukemia has also been shown to express CD103. The expression of CD103 on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as an αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV-1 induced human T cell line MAPS16
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western Blotting¹, immunoprecipitation¹, and immunohistochemical staining of frozen tissue sections¹.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106323 (BioLegend Cat. No. 285063)

Antigen Details

Structure: Type I transmembrane glycoprotein, integrin family; can be cleaved into 150 kD and 25 kD chains; associated with β7 integrin
Distribution: Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells; expressed on hairy cell leukemia
Function: Retention and activation of CD103⁺ lymphocytes in the intestinal epithelium, regulation of tissue-specific T cell homing
Ligand/Receptor: E-Cadherin
Cell Targets: Integrin β7
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Parker CM, et al. 1992. P. Natl. Acad. Sci. USA 89:1924.
2. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636.
3. Schon MP, et al. 1999. J. Immunol. 162:6641.
4. Shaw SK, et al. 1994. J. Biol. Chem. 269:6016.
Gene ID: 3682 View all products for this Gene ID
UniProt: View information about CD103 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD103 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD103 (Integrin αE)	Ber-ACT8	FC,WB,IP,IHC-F
FITC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Alexa Fluor® 488 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Alexa Fluor® 647 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Cyanine7 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 421™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
APC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 605™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Biotin anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 711™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Dazzle™ 594 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PerCP/Cyanine5.5 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Brilliant Violet 785™ anti-human CD103 (Integrin αE)	Ber-ACT8	FC
APC/Cyanine7 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
TotalSeq™-A0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
TotalSeq™-C0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
TotalSeq™-B0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
APC/Fire™ 750 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Fire™ 700 anti-human CD103 (Integrin αE) Antibody	Ber-ACT8	FC
TotalSeq™-D0145 anti-human CD103 (Integrin αE)	Ber-ACT8	PG
PE/Fire™ 640 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE anti-human CD103	Ber-ACT8	FC
FITC anti-human CD103	Ber-ACT8	FC
APC anti-human CD103	Ber-ACT8	FC
Spark YG™ 581 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark NIR™ 685 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark Blue™ 550 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
PE/Fire™ 810 anti-human CD103 (Integrin αE)	Ber-ACT8	FC
GMP FITC anti-human CD103 (Integrin αE)	Ber-ACT8	FC
GMP PE anti-human CD103 (Integrin αE)	Ber-ACT8	FC
Spark Red™ 718 anti-human CD103 (Integrin αE) (Flexi-Fluor™)	Ber-ACT8	FC
Spark Blue™ 574 anti-human CD103 (Integrin αE) (Flexi-Fluor™)	Ber-ACT8	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.