Spark Red™ 718 anti-human CD95 (Fas) Antibody, CD95, DX2

Pricing & Availability

Clone: DX2 (See other available formats)
Regulatory Status: RUO
Workshop: VI C-64
Other Names: Fas, APO-1, TNFRSF6
Isotype: Mouse IgG1, κ
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Product Citations: publications

DX2_Spark-Red-718_CD95_Antibody_071724 — Human peripheral blood lymphocytes were stained with anti-human CD95 (Fas) (clone DX2) Spark Red™ 718 (filled histogram). Open histogram represents unstained cells.

Compare all formats See Spark Red™ 718 spectral data

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305673

25 tests

£89

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305674

100 tests

£189

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Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD95 transfected L cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis³ (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining^4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656).

Note: EOS9.1 antibody can induce apoptosis without cross-linking.
RRID: AB_3662215 (BioLegend Cat. No. 305673)
AB_3662215 (BioLegend Cat. No. 305674)

Antigen Details

Structure: TNFR superfamily, type I transmembrane protein, 45 kD
Distribution: T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes
Function: Mediates apoptosis
Ligand/Receptor: Fas ligand (CD178)
Cell Type: B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.
Gene ID: 355 View all products for this Gene ID
UniProt: View information about CD95 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
APC anti-human CD95 (Fas)	DX2	FC
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PE anti-human CD95 (Fas)	DX2	FC
PE/Cyanine5 anti-human CD95 (Fas)	DX2	FC
Purified anti-human CD95 (Fas)	DX2	FC,ICC,IHC
Alexa Fluor® 488 anti-human CD95 (Fas)	DX2	FC
Alexa Fluor® 647 anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 421™ anti-human CD95 (Fas)	DX2	FC
Pacific Blue™ anti-human CD95 (Fas)	DX2	FC
PE/Cyanine7 anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 605™ anti-human CD95 (Fas)	DX2	FC
PerCP/Cyanine5.5 anti-human CD95 (Fas)	DX2	FC
Purified anti-human CD95 (Fas) (Maxpar® Ready)	DX2	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD95 (Fas)	DX2	FC
APC/Fire™ 750 anti-human CD95 (Fas)	DX2	FC
APC/Cyanine7 anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 510™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 711™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 785™ anti-human CD95 (Fas)	DX2	FC
Brilliant Violet 650™ anti-human CD95 (Fas)	DX2	FC
Alexa Fluor® 700 anti-human CD95 (Fas)	DX2	FC
TotalSeq™-A0156 anti-human CD95 (Fas)	DX2	PG
TotalSeq™-C0156 anti-human CD95 (Fas)	DX2	PG
TotalSeq™-B0156 anti-human CD95 (Fas)	DX2	PG
Ultra-LEAF™ Purified anti-human CD95 (Fas)	DX2	FC,ICC,IHC,Apop
TotalSeq™-D0156 anti-human CD95 (Fas)	DX2	PG
PE/Fire™ 640 anti-human CD95 (Fas)	DX2	FC
KIRAVIA Blue 520™ anti-human CD95 (Fas)	DX2	FC
APC/Fire™ 810 anti-human CD95 (Fas) Antibody	DX2	FC
PE anti-human CD95	DX2	FC
Spark YG™ 593 anti-human CD95 (Fas)	DX2	FC
PE/Fire™ 700 anti-human CD95 (Fas)	DX2	FC
PerCP/Fire™ 780 anti-human CD95 (Fas)	DX2	FC
Spark Blue™ 574 anti-human CD95 (Fas)	DX2	FC
Spark Red™ 718 anti-human CD95 (Fas)	DX2	FC
GMP PE anti-human CD95 (Fas)	DX2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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