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Spark Violet™ 538 anti-human CD45

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone
HI30
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
HI30_SPKVL-538_CD45_ASR_Antibody_111921
Typical results from human peripheral blood lymphocytes stained either with HI30 Spark Violet™ 538 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • HI30_SPKVL-538_CD45_ASR_Antibody_111921
    Typical results from human peripheral blood lymphocytes stained either with HI30 Spark Violet™ 538 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See Spark Violet™ 538 spectral data
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982320 500 µL £312
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Formulation
Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
Concentration
400 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

Suggested for Flow Cytometry
Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
  6. Avoid prolonged exposure of the reagent or stained cells to light.
  7. Spark Violet™ 538 conjugates may interact with biomolecules present in whole blood samples from certain donors that have been exposed to Polyethylene glycol (PEG) or similar molecules. The interpretation of the staining results must be evaluated by a qualified professional using the appropriate controls.

Antigen Details

Antigen References
  1. Thomas ML. 1989. Annu Rev Immunol. 7:339.
  2. Trowbridge IS, et al. 1994. Annu Rev Immunol.12:85.
Go To Top Version: 2    Revision Date: 02/21/2024

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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