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Spark Violet™ 538 anti-mouse CD8a Recombinant Antibody

Pricing & Availability
Clone
QA17A07 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA17A07_SPKVL538_CD8a_Antibody_122220.png
C57BL/6 mouse splenocytes were stained with CD3ε APC and CD8a recombinant (clone QA17A07) Spark Violet™ 538 (left) or mouse IgG1, κ Spark Violet™ 538 isotype control (right).
  • QA17A07_SPKVL538_CD8a_Antibody_122220.png
    C57BL/6 mouse splenocytes were stained with CD3ε APC and CD8a recombinant (clone QA17A07) Spark Violet™ 538 (left) or mouse IgG1, κ Spark Violet™ 538 isotype control (right).
Compare all formats See Spark Violet™ 538 spectral data
Cat # Size Price Quantity Check Availability Save
155019 25 µg £94
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155020 100 µg £193
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 538 has a maximum excitation of 396 nm and a maximum emission of 538 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2890706 (BioLegend Cat. No. 155019)
AB_2890706 (BioLegend Cat. No. 155020)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay AN, Brown MH. 1997. Biochem. Soc. Trans. 25:224-8.
  2. Zamoyska R. 1994. Immunity 1:243.
  3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 1    Revision Date: 12/22/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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