Spark YG™ 570 anti-HMB-45 Antibody, HMB-45, HMB-45

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Clone: HMB-45 (See other available formats)
Regulatory Status: RUO
Other Names: Melanoma Marker antibody, Human Melanoma Black, Melanocyte protein PMEL, PMEL
Isotype: Mouse IgG1, κ
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Product Citations: publications

HMB-45_Spark-YG-570_HMB-45_Antibody_081822 — Human paraffin-embedded melanoma tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Spark YG™ 570 anti-HMB-45 (clone HMB-45) (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue).

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911511

25 µg

£81

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911512

100 µg

£201

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Description

The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2924627 (BioLegend Cat. No. 911511)
AB_2924627 (BioLegend Cat. No. 911512)

Antigen Details

Structure: HMB-45 (Human Melanoma Black) is a monoclonal antibody that reacts with an antigen known as Pmel 17 present in melanocytic tumors such as melanomas.
Distribution: Tissue: expressed mainly in pigment cells of the skin, hair and eyes.
Distribution: melanocytes, intracellular.
Gene ID: 6490 View all products for this Gene ID
UniProt: View information about HMB-45 on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Description	Clone	Applications

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Other Formats

View All HMB-45 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-HMB-45	HMB-45	IHC-P,IHC-F
Alexa Fluor® 647 anti-HMB-45	HMB-45	IHC-P
Alexa Fluor® 594 anti-HMB-45	HMB-45	IHC-P
Alexa Fluor® 488 anti-HMB-45	HMB-45	IHC-P
Spark YG™ 570 anti-HMB-45	HMB-45	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/18/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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