- Clone
- HMB-45 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Melanoma Marker antibody, Human Melanoma Black, Melanocyte protein PMEL, PMEL
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HMB-45_Spark-YG-570_HMB-45_Antibody_081822 HMB-45_Spark-YG-570_HMB-45_Antibody_081822](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HMB-45_Spark-YG-570_HMB-45_Antibody_081822-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human paraffin-embedded melanoma tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Spark YG™ 570 anti-HMB-45 (clone HMB-45) (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
911511 | 25 µg | £81 | ||||
911512 | 100 µg | £201 |
The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm. - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Shen HQ, et al. 2013. Rom. J. Morphol. Embryol. 54(3):643û7 PubMed
- RRID
-
AB_2924627 (BioLegend Cat. No. 911511)
AB_2924627 (BioLegend Cat. No. 911512)
Antigen Details
- Structure
- HMB-45 (Human Melanoma Black) is a monoclonal antibody that reacts with an antigen known as Pmel 17 present in melanocytic tumors such as melanomas.
- Distribution
-
Tissue: expressed mainly in pigment cells of the skin, hair and eyes.
Distribution: melanocytes, intracellular. - Gene ID
- 6490 View all products for this Gene ID
- UniProt
- View information about HMB-45 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All HMB-45 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-HMB-45 | HMB-45 | IHC-P,IHC-F |
Alexa Fluor® 647 anti-HMB-45 | HMB-45 | IHC-P |
Alexa Fluor® 594 anti-HMB-45 | HMB-45 | IHC-P |
Alexa Fluor® 488 anti-HMB-45 | HMB-45 | IHC-P |
Spark YG™ 570 anti-HMB-45 | HMB-45 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-HMB-45
IHC staining of anti-HMB-45 antibody (clone HMB-45) on forma... -
Alexa Fluor® 647 anti-HMB-45
IHC staining of Alexa Fluor® 647 anti-HMB-45 antibody (clone... IHC staining of Alexa Fluor® 647 anti-HMB-45 antibody (clone... -
Alexa Fluor® 594 anti-HMB-45
Human paraffin-embedded melanoma tissue slices were prepared... Human paraffin-embedded prostate tissue slices were prepared... -
Alexa Fluor® 488 anti-HMB-45
Human paraffin-embedded melanoma tissue slices were prepared... Human paraffin-embedded prostate tissue slices were prepared... -
Spark YG™ 570 anti-HMB-45
Human paraffin-embedded melanoma tissue slices were prepared...
Follow Us