- Clone
- HMB-45 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Melanoma Marker antibody, Human Melanoma Black, Melanocyte protein PMEL, PMEL
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HMB-45_A488_Antibody_033122 HMB-45_A488_Antibody_033122](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HMB-45_A488_Antibody_033122.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded melanoma tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 488 anti-HMB-45 (clone HMB-45) (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). -
Human paraffin-embedded prostate tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 488 anti-HMB-45 (clone HMB-45) (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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911509 | 25 µg | £117 |
The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Shen HQ, et al. 2013. Rom. J. Morphol. Embryol. 54(3):643û7 PubMed
- RRID
-
AB_2924626 (BioLegend Cat. No. 911509)
Antigen Details
- Structure
- HMB-45 (Human Melanoma Black) is a monoclonal antibody that reacts with an antigen known as Pmel 17 present in melanocytic tumors such as melanomas.
- Distribution
-
Tissue: expressed mainly in pigment cells of the skin, hair and eyes.
Distribution: melanocytes, intracellular. - Gene ID
- 6490 View all products for this Gene ID
- UniProt
- View information about HMB-45 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All HMB-45 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-HMB-45 | HMB-45 | IHC-P,IHC-F |
Alexa Fluor® 647 anti-HMB-45 | HMB-45 | IHC-P |
Alexa Fluor® 594 anti-HMB-45 | HMB-45 | IHC-P |
Alexa Fluor® 488 anti-HMB-45 | HMB-45 | IHC-P |
Spark YG™ 570 anti-HMB-45 | HMB-45 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-HMB-45
IHC staining of anti-HMB-45 antibody (clone HMB-45) on forma... -
Alexa Fluor® 647 anti-HMB-45
IHC staining of Alexa Fluor® 647 anti-HMB-45 antibody (clone... IHC staining of Alexa Fluor® 647 anti-HMB-45 antibody (clone... -
Alexa Fluor® 594 anti-HMB-45
Human paraffin-embedded melanoma tissue slices were prepared... Human paraffin-embedded prostate tissue slices were prepared... -
Alexa Fluor® 488 anti-HMB-45
Human paraffin-embedded melanoma tissue slices were prepared... Human paraffin-embedded prostate tissue slices were prepared... -
Spark YG™ 570 anti-HMB-45
Human paraffin-embedded melanoma tissue slices were prepared...
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