TotalSeq™-D0161 anti-human CD11b antibody, CD11b, ICRF44

Pricing & Availability

Clone: ICRF44 (See other available formats)
Regulatory Status: RUO
Workshop: IV M047
Other Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype: Mouse IgG1, κ
Barcode Sequence: GACAAGTGATCTGCA
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Product Citations: publications

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301363

10 µg

£253

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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as α_M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β₂ (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Chimpanzee, Common Marmoset, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.
RRID: AB_2892345 (BioLegend Cat. No. 301363)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 165-170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor: ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
Gene ID: 3684 View all products for this Gene ID
UniProt: View information about CD11b on UniProt.org

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Other Formats

View All CD11b Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD11b	ICRF44	FC
Biotin anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
PE/Cyanine5 anti-human CD11b	ICRF44	FC
Purified anti-human CD11b	ICRF44	FC,CyTOF®,ICC,IHC
Pacific Blue™ anti-human CD11b	ICRF44	FC
Alexa Fluor® 488 anti-human CD11b	ICRF44	FC
Alexa Fluor® 647 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Brilliant Violet 421™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 570™ anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
Brilliant Violet 605™ anti-human CD11b	ICRF44	FC
Brilliant Violet 510™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 650™ anti-human CD11b	ICRF44	FC
Purified anti-human CD11b (Maxpar® Ready)	ICRF44	FC,CyTOF®
Alexa Fluor® 594 anti-human CD11b	ICRF44	ICC,FC
APC/Cyanine7 anti-human CD11b	ICRF44	FC
Brilliant Violet 711™ anti-human CD11b	ICRF44	FC
Brilliant Violet 785™ anti-human CD11b	ICRF44	FC
PE/Dazzle™ 594 anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
APC anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
TotalSeq™-A0161 anti-human CD11b	ICRF44	PG
Alexa Fluor® 700 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
TotalSeq™-B0161 anti-human CD11b	ICRF44	PG
TotalSeq™-C0161 anti-human CD11b	ICRF44	PG
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Ultra-LEAF™ Purified anti-human CD11b	ICRF44	FC,CyTOF®,Block,ICC,IHC
TotalSeq™-D0161 anti-human CD11b	ICRF44	PG
GMP PE/Cyanine7 anti-human CD11b	ICRF44	FC
Pacific Blue™ anti-human CD11b	ICRF44	FC
GMP PE anti-human CD11b	ICRF44	FC
Spark UV™ 387 anti-human CD11b	ICRF44	FC
GMP PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
GMP APC anti-human CD11b	ICRF44	FC
GMP FITC anti-human CD11b	ICRF44	FC
GMP APC/Fire™ 750 anti-human CD11b	ICRF44	FC
Spark Blue™ 515 anti-human CD11b	ICRF44	FC
Spark Violet™ 500 anti-human CD11b	ICRF44	FC
Spark Red™ 718 anti-human CD11b (Flexi-Fluor™)	ICRF44	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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