Ultra-LEAF™ Purified anti-human CD51 Antibody, CD51, NKI-M9

Pricing & Availability

Clone: NKI-M9 (See other available formats)
Regulatory Status: RUO
Workshop: IV 103
Other Names: Vitronectin receptor α chain, Integrin αV, αV integrin, ITGAV
Isotype: Mouse IgG2a, κ
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Product Citations: publications

NKI-M9_LEAF_020808 — Human melanoma cell line M21 stained with Ultra-LEAF™ purified NKI-M9, followed by anti-mouse IgG FITC

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327911

100 µg

£185

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327912

1 mg

£464

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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD51 is a type I integral membrane glycoprotein, known as vitronectin receptor α chain, or integrin α_V. It forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Melonama cells
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
ICC - Verified
ELISA, Block, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2832640 (BioLegend Cat. No. 327911)
AB_2832640 (BioLegend Cat. No. 327912)

Antigen Details

Structure: Integrin, forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8, 125 kD and 24 kD in reduced condition, 150 kD (unreduced)
Distribution: Endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.
Function: Adhesion, angiogenesis
Ligand/Receptor: Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen, fibronectin, laminin.
Cell Type: Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Osteoclasts, Platelets
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
Gene ID: 3685 View all products for this Gene ID
UniProt: View information about CD51 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD51 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD51	NKI-M9	FC,ICC,ELISA,Block,IP
Biotin anti-human CD51	NKI-M9	FC
FITC anti-human CD51	NKI-M9	FC
PE anti-human CD51	NKI-M9	FC
Ultra-LEAF™ Purified anti-human CD51	NKI-M9	FC,ICC,ELISA,Block,IP
APC anti-human CD51	NKI-M9	FC
PE/Cyanine7 anti-human CD51	NKI-M9	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/23/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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