- Clone
- 5H10-27(MFR5) (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α5 integrin, VLA-5 α chain, Integrin α5, Fnra, Itga5
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
103817 | 100 µg | £185 | ||||
103818 | 1 mg | £509 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- (C57BL/6 x A/J)F1 mouse mast cell line
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-ß1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103817 and 103818).
-
Application References
(PubMed link indicates BioLegend citation) -
- Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
- Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
- Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
- Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
- Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
- Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
- Cuklerman E, et al. 2001. Science 294:1708. (IHC)
- Jia W, et al. 2005. Blood 106:3854. (FC)
- RRID
-
AB_2832318 (BioLegend Cat. No. 103817)
AB_2832318 (BioLegend Cat. No. 103818)
Antigen Details
- Structure
- Integrin family, associates with integrin β1, 135 kD
- Distribution
-
Thymocytes, activated T cells, mast cells
- Function
- Adhesion, costimulation
- Ligand/Receptor
- Fibronectin
- Cell Type
- Mast cells, Mesenchymal Stem Cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365. - Gene ID
- 16402 View all products for this Gene ID
- UniProt
- View information about CD49e on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD49e Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD49e | 5H10-27(MFR5) | FC |
Purified anti-mouse CD49e | 5H10-27(MFR5) | FC,IHC-F |
Alexa Fluor® 488 anti-mouse CD49e | 5H10-27(MFR5) | FC |
Alexa Fluor® 647 anti-mouse CD49e | 5H10-27(MFR5) | FC |
PE/Cyanine7 anti-mouse CD49e | 5H10-27(MFR5) | FC |
APC anti-mouse CD49e | 5H10-27(MFR5) | FC |
Ultra-LEAF™ Purified anti-mouse CD49e | 5H10-27(MFR5) | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us