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Alexa Fluor® 647 anti-mouse IgG1 Antibody

Pricing & Availability
Clone
RMG1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G1
Isotype
Rat IgG
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Product Citations
publications
A_RMG1-1_AF647_IgG1_Antibody_FC_1_041714.jpg
Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone UCHT1, mouse IgG1 isotype, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) Alexa Fluor® 647.
  • A_RMG1-1_AF647_IgG1_Antibody_FC_1_041714.jpg
    Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone UCHT1, mouse IgG1 isotype, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) Alexa Fluor® 647.
  • B_RMG1-1_A647_IgG1_IHCF_101218
    FVB/N mouse frozen thymus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-rat CD90/mouse CD90.1 (clone OX-7) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 Goat anti-mouse IgG1 (clone RMG1-1) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • C_RMG1-1_A647_IgG1_IHCP_062123
    IHC staining of Alexa Fluor® 647 anti-mouse IgG1 on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 5 µg/mL of purified mouse anti-CD8a (Cat. No. 372902) at 4°C. This was followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG1 (clone RMG1-1) at 2 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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406617 25 µg £61
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406618 100 µg £133
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Description

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed
  2. Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations
  1. Stabel JR, et al. 2022. PLoS One. 17:e0278313. PubMed
  2. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  3. Zbinden A, et al. 2021. Adv Sci (Weinh). 2002500:8. PubMed
  4. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  5. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  6. Jennewein MF, et al. 2020. Cell. 178(1):202-215.e14.. PubMed
  7. Nguyen T, et al. 2017. Nat Immunol. 18:321-333. PubMed
  8. Bhushan S, et al. 2015. J Immunol. 194:5455. PubMed
RRID
AB_2563476 (BioLegend Cat. No. 406617)
AB_2563476 (BioLegend Cat. No. 406618)

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Go To Top Version: 4    Revision Date: 06/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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