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APC anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD56_APC_020808
Human peripheral blood lymphocytes stained with HCD56 APC
  • HCD56_APC_020808
    Human peripheral blood lymphocytes stained with HCD56 APC
Compare all formats See APC spectral data
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318309 25 tests £102
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318310 100 tests £215
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
  2. Kenney DJ, et al. 2022. Cell Rep. 39:110714. PubMed
  3. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  4. Chen L, et al. 2023. Immunology. 169:204. PubMed
  5. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
  6. De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed
  7. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  8. Siebert N, et al. 2014. PLoS One. 9:107692. PubMed
  9. Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed
  10. Davis Z, et al. 2011. J Vis Exp. 49: 2668. PubMed
  11. Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
  12. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  13. Shokri MR, et al. 2019. Sci Rep. 9:10007. PubMed
  14. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  15. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  16. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
  17. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  18. Snyder J, et al. 2014. PLoS One. 9:107257. PubMed
  19. Miguel Reina‐Campos et al. 2019. Cancer cell. 35(3):385-400 . PubMed
  20. Robin J, et al. 2015. J Vis Exp. 95: 52307. PubMed
  21. Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed
  22. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  23. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  24. Pakula A, et al. 2018. Methods Mol Biol. 1889:01:00. PubMed
  25. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed
  26. Pereira PMR, et al. 2022. Nat Commun. 13:2526. PubMed
  27. Andersen J, et al. 2020. Cell. 183:1913. PubMed
  28. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  29. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  30. Ames E, et al. 2015. J Immunol. 195: 4010 - 4019. PubMed
  31. Rider P, et al. 2015. J Immunol. 195: 1705-1712. PubMed
  32. Smith SA, et al. 2019. Front Immunol. 9:3163. PubMed
  33. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  34. Rosental B, et al. 2011. J Immunol. 187:5693. PubMed
  35. Al Tanoury Z, et al. 2020. Development. 147:00:00. PubMed
  36. Vabret N, et al. 2022. iScience. 25:104599. PubMed
  37. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  38. Ma Y, et al. 2020. Cell Prolif. 53:e12802. PubMed
  39. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  40. Yan W, et al. 2020. Biology of Reproduction. . PubMed
  41. Schlecker E, et al. 2014. Cancer Res. 74:3429. PubMed
  42. Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed
  43. Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
  44. Taouk G, et al. 2019. Sci Rep. 9:8756. PubMed
  45. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  46. Correia DV, et al. 2011. Blood. 118:992. PubMed
  47. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  48. Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
  49. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  50. Al Tanoury Z, et al. 2020. Development. . PubMed
  51. Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed
  52. Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed
  53. Strijker JGM, et al. 2021. J Pers Med. 11:. PubMed
RRID
AB_604106 (BioLegend Cat. No. 318309)
AB_604106 (BioLegend Cat. No. 318310)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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