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APC anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
C068C2_APC_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • C068C2_APC_2_082911
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141707 25 µg £90
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141708 100 µg £203
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Liu J, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.139. PubMed
  2. Wang C, et al. 2018. J Pharmacol Exp Ther. 365:72. PubMed
  3. Jiang Z, et al. 2021. J Clin Invest. 131: . PubMed
  4. Kiepura A, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  6. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  7. Miki H, et al. 2020. J Immunol. 204:1892. PubMed
  8. Zhou Y, et al. 2022. Br J Pharmacol. 179:1978. PubMed
  9. Liang X, et al. 2022. Cancer Commun (Lond). 42:205. PubMed
  10. Chang S, et al. 2022. Phytother Res. 36:1678. PubMed
  11. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  12. Wu J, et al. 2023. Bioact Mater. 20:259. PubMed
  13. Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed
  14. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  15. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  16. Lin J, et al. 2022. Eur J Histochem. 67: . PubMed
  17. Sun J, et al. 2023. Mediators Inflamm. 2022:1108569. PubMed
  18. Ye Z, et al. 2023. Front Med (Lausanne). 9:1075465. PubMed
  19. De Schepper S, et al. 2023. Nat Neurosci. 26:406. PubMed
  20. Kimura Y, et al. 2023. Front Immunol. 14:1070196. PubMed
  21. Liang P, et al. 2022. Prostate Cancer Prostatic Dis. 25:539. PubMed
  22. Gao T, et al. 2022. Cell Biochem Biophys. 80:579. PubMed
  23. Jia DJ, et al. 2022. Gut Microbes. 14:2145843. PubMed
  24. Song X, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. Wu Y, et al. 2023. Adv Mater. 35:e2210787. PubMed
  26. Sheng Y, et al. 2023. Cancers (Basel). 15:. PubMed
  27. Wang Y, et al. 2023. Front Bioeng Biotechnol. 11:1128268. PubMed
  28. Kaur N, et al. 2023. Heliyon. 9:e14952. PubMed
  29. Lu Y, et al. 2023. J Transl Med. 21:339. PubMed
  30. Zhang Y, et al. 2023. NPJ Regen Med. 8:28. PubMed
  31. Pathak JL, et al. 2021. Front Cell Dev Biol. 628139:9. PubMed
  32. Jeong J, et al. 2017. Sci Rep. 7:39848. PubMed
  33. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  34. Liu C, et al. 2019. J Transl Med. 17:300. PubMed
  35. Zhang X, et al. 2017. J Cell Mol Med. 10.1111/jcmm.13329. PubMed
  36. Hong M, et al. 2021. Int J Mol Sci. 22:. PubMed
  37. El-Hindi K, et al. 2022. Int J Mol Sci. 23:. PubMed
  38. He J, et al. 2022. Front Immunol. 12:760138. PubMed
  39. Giordano C, et al. 2014. Hum Mol Genet. . PubMed
  40. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  41. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  42. Tahiri H, et al. 2016. Sci Rep. 6:37391. PubMed
  43. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  44. Chang CF, et al. 2017. Neurobiol Dis. 103:54. PubMed
  45. Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed
  46. Zhang J, et al. 2021. Front Cardiovasc Med. 8:737652. PubMed
  47. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  48. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  49. Adachi Y, et al. 2022. Nat Commun. 13:5117. PubMed
  50. Yuan Q, et al. 2021. Cell Reports. 34(5):108724. PubMed
  51. Young JA, et al. 2020. Journal of Neuroendocrinology. e12854.. PubMed
  52. Shang W, et al. 2019. J Diabetes Res. 2019:5641271. PubMed
  53. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  54. Zhang L, et al. 2022. Acta Pharm Sin B. 12:3427. PubMed
  55. Chen X, et al. 2021. Cell Death Differ. 28:1237. PubMed
  56. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
  57. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  58. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  59. Motoyama S, et al. 2020. Cells. :9. PubMed
  60. Li S, et al. 2021. Ann Transl Med. 9:1644. PubMed
  61. Chen F, et al. 2022. Evid Based Complement Alternat Med. 2022:1240242. PubMed
  62. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  63. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  64. Li Q, et al. 2021. Brain Behav Immun. 94:437. PubMed
  65. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  66. Qiu J, et al. 2016. Sci Rep. 6:39407. PubMed
  67. Lasky C, et al. 2015. Infect Immun . 83: 2627 - 2635. PubMed
  68. van Dierendonck XAMH, et al. 2020. Cell Reports. 30(6):1811-1822. PubMed
  69. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  70. Yan J, et al. 2018. Nat Commun. 9:33. PubMed
  71. Kan C, et al. 2022. Bone Res. 10:62. PubMed
  72. Liao Y, et al. 2020. Mol Ther. 28:855. PubMed
  73. Hu M, et al. 2022. Can Respir J. 2022:5802938. PubMed
  74. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  75. Xie Z, et al. 2021. Open Life Sci. 16:653. PubMed
  76. Wu L, et al. 2022. Front Cell Dev Biol. 9:684842. PubMed
  77. Chen X, et al. 2022. J Appl Oral Sci. 30:e20220316. PubMed
  78. Taguchi K, et al. 2015. J Urol. . PubMed
  79. Calvo B, et al. 2020. IBRO Rep. 8:36. PubMed
  80. Jin RM, et al. 2017. J Immunol. 198:352. PubMed
  81. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  82. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  83. Xiao Y, et al. 2022. Clin Transl Med. 12:e1071. PubMed
  84. Feng T, et al. 2022. Nat Commun. 13:5208. PubMed
  85. Kornstädt L, et al. 2021. Front Immunol. 11:607048. PubMed
  86. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  87. Taguchi K, et al. 2014. J Am Soc Nephrol. 25:1680. PubMed
  88. Rios-Doria J, et al. 2021. Front Oncol. 10:598477. PubMed
  89. Ayala TS, et al. 2019. Sci Rep. 9:11447. PubMed
  90. Mallampalli RK, et al. 2021. Front Immunol. 12:735576. PubMed
  91. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  92. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
  93. Lee JY, et al. 2020. Nat Commun. 11:2358. PubMed
  94. Yoon J, et al. 2020. Front Cell Dev Biol. 0.520138889. PubMed
  95. Kumar MP, et al. 2018. Cell Rep. 25:1458. PubMed
  96. Wang Q, et al. 2018. Nat Commun. 9:559. PubMed
  97. Xu M, et al. 2022. Genes Dis. 9:429. PubMed
  98. Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed
  99. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  100. Moya-Pérez A, et al. 2015. PLoS One. 10: 0126976. PubMed
  101. De Simone G, et al. 2021. Immunity. :. PubMed
  102. Rhys HI, et al. 2018. EBioMedicine. 29:60. PubMed
  103. Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
  104. Zhou X, et al. 2022. Front Pharmacol. 13:872188. PubMed
  105. Blriot C, et al. 2021. Immunity. :. PubMed
  106. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  107. Yamakawa D, et al. 2021. Cell Reports. 34(10):108817. PubMed
  108. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  109. Sun S, et al. 2021. Front Immunol. 12:777665. PubMed
  110. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  111. Zhang S, et al. 2021. J Inflamm Res. 14:4915. PubMed
  112. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
  113. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  114. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  115. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  116. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  117. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  118. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  119. Remmerie A, et al. 2020. Immunity. 53:641. PubMed
  120. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  121. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  122. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
  123. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  124. Dong R, et al. 2021. Frontiers in Immunology. 12:620238. PubMed
  125. Zhang Y, et al. 2020. Mol Med Rep. 22:534. PubMed
  126. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  127. Guo D, et al. 2022. Bone Res. 10:45. PubMed
  128. Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed
  129. Li ZL, et al. 2021. Mol Ther Nucleic Acids. 25:502. PubMed
  130. He H, et al. 2012. Blood. 120:3152. PubMed
  131. Dai K, et al. 2015. J Leukoc Biol. 98: 1071 - 1080. PubMed
  132. Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed
  133. Gao Z, et al. 2022. J Inflamm Res. 15:3337. PubMed
  134. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
  135. Li K, et al. 2020. J Cell Mol Med. 24:13115. PubMed
  136. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  137. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  138. Rao L, et al. 2020. Nat Commun. 11:4909. PubMed
  139. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  140. Nam J, et al. 2021. Adv Ther (Weinh). 4:. PubMed
  141. Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed
  142. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  143. Roux C, et al. 2021. Antioxidants (Basel). 10:. PubMed
  144. Tsunekuni K, et al. 2020. J Clin Med. 9:00. PubMed
  145. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  146. Lee SH, et al. 2018. J Exp Med. 215:357. PubMed
  147. Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed
  148. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  149. Cui Y, et al. 2022. Front Oncol. 12:732860. PubMed
  150. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  151. Canan C, et al. 2014. J Leukoc Biol. 96:473. PubMed
  152. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  153. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  154. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  155. Catrysse L, et al. 2021. Cell Rep. 36:109748. PubMed
  156. Zhu Y, et al. 2021. Int J Oral Sci. 13:25. PubMed
  157. Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
  158. Baardman J et al. 2018. Cell reports. 25(8):2044-2052 . PubMed
  159. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  160. Durga Devi T, et al. 2017. Am J Pathol. 187:2659. PubMed
  161. Kurelac I, et al. 2019. J Clin Med. 1.85. PubMed
  162. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  163. Song HY, et al. 2021. Mol Ther. S1525-0016:00472-X. PubMed
  164. Luo ZW, et al. 2021. Int J Nanomedicine. 16:2949. PubMed
  165. Liu Y, et al. 2019. Am J Physiol Cell Physiol. 317:C762. PubMed
  166. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
  167. Nasti A, et al. 2017. Eur J Immunol. 47:2163. PubMed
  168. Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
  169. Ding T, et al. 2021. J Nanobiotechnology. 19:247. PubMed
  170. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
RRID
AB_10896057 (BioLegend Cat. No. 141707)
AB_10896057 (BioLegend Cat. No. 141708)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 3    Revision Date: 04/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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