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Brilliant Violet 421™ anti-human CD337 (NKp30) Antibody

Pricing & Availability
Clone
P30-15 (See other available formats)
Regulatory Status
RUO
Other Names
NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P30-15_BV421_CD337_Antibody_062719.png
Human peripheral blood lymphocytes were stained with CD56 FITC and CD337 (clone P30-15) Brilliant Violet 421™ (left) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (right) and CD56 FITC
  • P30-15_BV421_CD337_Antibody_062719.png
    Human peripheral blood lymphocytes were stained with CD56 FITC and CD337 (clone P30-15) Brilliant Violet 421™ (left) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (right) and CD56 FITC
Compare all formats See Brilliant Violet 421™ spectral data
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325227 25 tests £121
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325228 100 tests £265
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Description

The p30-15 monoclonal antibody recognizes CD337 also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3. NKp30 is a type I transmembrane protein, member of the natural cytotoxicity receptor family that contains one immunoglobulin-like domain. NKp30 has an apparent molecular weight of 30 kD and six isoforms are produced by alternative splicing. NKp30 is expressed on resting and activated NK cells. NKp30 enhances NK cell cytolysis of tumor cellts that are deficient in MHC class I molecules. NKp30 has been shown to associate with CD59 and TCRζ. The p30-15 antibody against human NKp30 has been shown to be useful for flow cytometry, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human NKp30
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA, stimulation of human NK cells via NKp30 in a redirected lysis assay, and blocking of NKp30 function in solution1,3,5. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 325223 & 325224).

Application References

(PubMed link indicates BioLegend citation)
  1. Stark S, et al. 2005. J. Immunol. Methods 296:149. (Block)
  2. Mansater I, et al. 2008. J. Immunol. 181:1869. PubMed
  3. Markel G, et al. 2009. PLOS One 4:e5597. (Block)
  4. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  5. Byrd A, et al. 2007. PLoS One. 2:e1339. (Block)
Product Citations
  1. Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
RRID
AB_2810488 (BioLegend Cat. No. 325227)
AB_2810488 (BioLegend Cat. No. 325228)

Antigen Details

Structure
Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain. Approximately 44 kD. Six isoforms produced by alternative splicing.
Distribution

IL-2 activated NK cells and NK cell lines, some γ/δ T cells activated in vitro

Function
NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
Interaction
CD59, TCRζ
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Pende D, et al. 1999. J. Exp. Med. 190:1505.

Gene ID
259197 View all products for this Gene ID
UniProt
View information about CD337 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06/27/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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