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Brilliant Violet 421™ anti-human CD34 Antibody

Pricing & Availability
Clone
561 (See other available formats)
Regulatory Status
RUO
Other Names
Gp105-120, My10, Mucosialin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
561_BV421_CD34_Antibody_1_091824
Human peripheral blood mononuclear cells were stained with CD14 PE, CD45 PerCP/Cyanine5.5, and CD34 (clone 561) Brilliant Violet 421™ (left) or mouse IgG2a Brilliant Violet 421™ isotype control (right). Dot plots are from gated on CD14-negative cell population.
  • 561_BV421_CD34_Antibody_1_091824
    Human peripheral blood mononuclear cells were stained with CD14 PE, CD45 PerCP/Cyanine5.5, and CD34 (clone 561) Brilliant Violet 421™ (left) or mouse IgG2a Brilliant Violet 421™ isotype control (right). Dot plots are from gated on CD14-negative cell population.
Compare all formats See Brilliant Violet 421™ spectral data
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343609 25 tests £140
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343610 100 tests £275
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.

Application References

(PubMed link indicates BioLegend citation)
  1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
  2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations
  1. Scala S, et al. 2023. Nat Commun. 14:3068. PubMed
  2. Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed
  3. Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed
  4. Yuzuriha A, et al. 2021. Methods Mol Biol. 2454:411. PubMed
  5. de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed
  6. Vasu S, et al. 2016. Blood. 127: 2879 - 2889. PubMed
  7. Brandsma AM, et al. 2021. Cancer Discov. . PubMed
  8. Tong J, et al. 2021. Cell Stem Cell. 28(3):502-513.e6. PubMed
  9. Lu YC, et al. 2018. Cell Rep. 25:2083. PubMed
  10. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  11. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  12. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  13. Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed
  14. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  15. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  16. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
  17. Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed
  18. Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed
  19. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  20. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
  21. Ward D, et al. 2016. Haematologica. 101: 286 - 296. PubMed
  22. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  23. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  24. Ainsworth RI, et al. 2022. Nat Commun. 13:6221. PubMed
RRID
AB_11147951 (BioLegend Cat. No. 343609)
AB_11147951 (BioLegend Cat. No. 343610)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selecin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 11/01/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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