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Purified anti-FOXA1 Antibody

Pricing & Availability
Clone
2F83 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein A1, HNF3α, Hepatic nuclear factor 3 α
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A-2F83_Pure_FOXA1_Antibody_012319
Whole cell extracts (15 µg protein) from HEL (negative control), HeLa (reduced expression control), HepG2, and NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-FOXA1 Antibody, clone 2F83, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat Anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal loading was confirmed by probing membranes with Direct Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) at a 1:50000 dilution. Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas
  • A-2F83_Pure_FOXA1_Antibody_012319
    Whole cell extracts (15 µg protein) from HEL (negative control), HeLa (reduced expression control), HepG2, and NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-FOXA1 Antibody, clone 2F83, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat Anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal loading was confirmed by probing membranes with Direct Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) at a 1:50000 dilution. Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas
  • B-2F83_Pure_FOXA1_Antibody_ICC_061919
    MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and permeabilized with 0.5% Triton X-100 buffer for 10 minutes at room temperature. Then blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS). The cells were stained with 5 µg/mL anti-FOXA1 (clone 2F83) purified at 4°C overnight. On the next day, cells were stained with Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) for an hour at room temperature. Finally, cells were incubated with Flash Phalloidin™ Green 488 (green) for 20 mintues at room temperature. Nuclei were counterstained with DAPI (blue). Images were acquired with a 40X objective.
  • C-2F83_Pure_FOXA1_Antibody_IHCP_061919
    Human paraffin-embedded prostate tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer pH 6.0 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permealized with 0.5% TritonX-100 for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human FOXA1 (clone 2F83) Purifed at 4°C overnight followed with one hour room temperature incubation of anti-mouse IgG Alexa Fluor® 594 (clone Poly4053, Red). Nuclei were counterstained with DAPI (Blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
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612001 25 µg £70
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612002 100 µg £174
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Description

FOXA1 is a transcriptional activator that is required for tissue-specific differentiation during embryonic development. It also functions as a coactivator with nuclear hormone receptors, including ESR1, and has been shown to play a major role in driving estrogen receptor positive breast cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human FOXA1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When using this clone for western blot, we highly recommend using lysate that has not been subjected to any freeze-thaw cycles.

Application References

(PubMed link indicates BioLegend citation)
  1. Besenard V, et al. 2004. Gene Expr Patterns. 5:193-208. (IHC-P)
RRID
AB_2801115 (BioLegend Cat. No. 612001)
AB_2801115 (BioLegend Cat. No. 612002)

Antigen Details

Structure
FOXA1 is a 472 amino acid protein with a predicted molecular weight of 49 kD.
Distribution

Nucleus/Early endoderm derived cells

Function
Transcriptional activator
Interaction
FOXA2, NKX2-1, Histones H3 and H4
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
3169 View all products for this Gene ID
UniProt
View information about FOXA1 on UniProt.org
Go To Top Version: 1    Revision Date: 01/23/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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