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Purified anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
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Product Citations
publications
1-MEL-14_Purified_090707
C57BL/6 mouse splenocytes stained with purified MEL-14, followed by anti-rat IgG FITC
  • 1-MEL-14_Purified_090707
    C57BL/6 mouse splenocytes stained with purified MEL-14, followed by anti-rat IgG FITC
  • 2-MEL-14_pure_CD62L_Antibody_2_103018
    Fresh, frozen mouse spleen was stained with purified CD62L clone MEL-14 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with Ly6c Cy3 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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104402 500 µg £95
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  2. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  3. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  4. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  5. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  6. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  7. Ban YH, et al. 2017. Cell Rep. 2.6375. PubMed
  8. Guo R, et al. 2020. Cell Res. 30:21. PubMed
  9. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  10. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  11. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  12. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  13. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  14. Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
  15. Muhammad F, et al. 2020. Front Immunol. 975:11. PubMed
  16. Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
  17. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  18. Delgobo M, et al. 2021. Front Immunol. 12:584538. PubMed
  19. Li W, et al. 2020. J Clin Invest. 130:6718. PubMed
  20. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  21. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  22. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  23. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  24. Oba T, et al. 2021. J Immunother Cancer. 9:. PubMed
  25. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  26. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  27. Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed
  28. Sato Y, et al. 2021. BMC Cancer. 21:1222. PubMed
  29. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  30. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
  31. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  32. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  33. Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed
  34. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  35. Gil-Pulido J, et al. 2017. PLoS One.. 10.1371/journal.pone.0181947. PubMed
  36. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  37. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  38. Kenna T, et al. 2008. Blood. 111:2091. PubMed
  39. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  40. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  41. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  42. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  43. Florek M, et al. 2015. PLoS One. 10: 0145763. PubMed
  44. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  45. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  46. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  47. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  48. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  49. Flietner E, et al. 2022. Sci Rep. 12:10616. PubMed
  50. Yi J, et al. 2019. Mol Cells. 42:228. PubMed
  51. Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
  52. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  53. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  54. Jun JC, et al. 2017. PLoS One. 12:e0180688. PubMed
  55. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  56. Karuppuchamy T, et al. 2020. Inflamm Bowel Dis. 216:26. PubMed
  57. Olver S, et al. 2005. Cancer Res. 66:571. PubMed
  58. Richards KA, et al. 2018. Front Immunol. 0.829861111. PubMed
  59. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  60. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  61. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  62. Managlia E, et al. 2019. Am J Pathol. 189:604. PubMed
  63. Fukushima A, et al. 2006. Invest Ophthalmol Vis Sci. 47:657. PubMed
  64. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  65. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
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RRID
AB_313089 (BioLegend Cat. No. 104402)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org
Go To Top Version: 2    Revision Date: 10/30/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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