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Purified anti-mouse/human Mac-2 (Galectin-3) Antibody

Pricing & Availability
Clone
M3/38 (See other available formats)
Regulatory Status
RUO
Other Names
Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype
Rat IgG2a, κ
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Product Citations
publications
M3_38_Pure_072808.jpg
BALB/c peritoneal macrophages stained with M3/38 purified, followed by anti-rat IgG FITC
  • M3_38_Pure_072808.jpg
    BALB/c peritoneal macrophages stained with M3/38 purified, followed by anti-rat IgG FITC
  • M3-38_Purified_Mac2_Antibody_ICC_011921
    HeLa cells were stained with purified anti-Galectin 3 (M3/38) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).
  • M3-38_Purified_Mac2_Antibody_IP_011921
    Immunoprecipitation/Western Blot analysis using anti‐mouse/human Mac‐2 (Galectin‐3) Antibody (clone M3/38). 150 µg of HeLa cell lysates were tested at a protein concentration of 1 µg/µL for each sample. Lane 1 was immunoprecipitated with a control antibody and lane 2 was immunoprecipitated with anti‐mouse/human Mac‐2 (Galectin‐3) Antibody (clone M3/38). Western blot analysis was performed using anti‐mouse/human Mac‐2 antibody (clone Gal397).
  • M3-38_Purified_Mac2_Antibody_WB_011921
    Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat cells (lane 2, 15 µg), 3T3-L1 (lane 3, 15 µg) and Raw264.7 (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse/human Mac-2 (Galectin-3) antibody (clone M3/38). Proteins were visualized using an HRP Goat anti-rat IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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125401 50 µg £50
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125402 500 µg £177
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Description

Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against galectin-3 of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, WB, IP - Verified
IHC, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-37.

Application References

(PubMed link indicates BioLegend citation)
  1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
  2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
  3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
  4. Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
  5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
  6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
  7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
  8. Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
  9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
  10. Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed
Product Citations
  1. Demotte N, et al. 2010. Cancer Res. 70:7476. PubMed
  2. Di Liberto G, et al. 2022. Brain. 145:2730. PubMed
  3. Ayyar BV, et al. 2023. Nat Commun. 14:1148. PubMed
  4. Zheng B, et al. 2023. Research (Wash D C). 6:0135. PubMed
  5. Sakamoto S, et al. 2023. Sci Rep. 13:9350. PubMed
  6. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  7. Thiemann S, et al. 2015. J Biol Chem. 290: 22662-22677. PubMed
  8. Kannt A, et al. 2021. Br J Pharmacol. 178:2412. PubMed
  9. Kaya T, et al. 2022. Nat Neurosci. 25:1446. PubMed
  10. Heitel P, et al. 2020. Commun Chem. 3:174. PubMed
  11. Jia J, et al. 2020. Dev Cell. 69:52. PubMed
  12. Zhou H, et al. 2016. Mol Cell Biol. 36: 2027 - 2038. PubMed
  13. Bu D, et al. 2019. JCI Insight. 4:e125094. PubMed
  14. Evans C, et al. 2010. Arterioscler Thromb Vasc Biol . 30:2443. PubMed
  15. Helmstädter M, et al. 2021. ACS Pharmacol Transl Sci. 4:966. PubMed
  16. Crewe C et al. 2018. Cell. 175(3):695-708 . PubMed
  17. Manich G, et al. 2020. Front Cell Neurosci. 14:567404. PubMed
  18. Eakin AJ, et al. 2020. Front Cell Dev Biol. 0.888194444. PubMed
  19. Davison LM, et al. 2021. Front Immunol. 12:681503. PubMed
  20. Møllerhøj MB, et al. 2022. Clin Transl Sci. 15:1167. PubMed
  21. Safaiyan S, et al. 2021. Neuron. 109(7):1100-1117.e10. PubMed
  22. Bosch-Queralt M, et al. 2021. Nat Metab. 3:211. PubMed
  23. Perakakis N, et al. 2021. Liver Int. 41:1853. PubMed
  24. Boland ML, et al. 2019. World J Gastroenterol. 25:4904. PubMed
  25. Li N, et al. 2021. eLife. 10:00. PubMed
  26. Perakakis N, et al. 2020. Hepatol Commun. 1.070833333. PubMed
  27. Roth JD, et al. 2019. Sci Rep. 9:9046. PubMed
  28. Bussi C, et al. 2018. J Cell Sci. 131. PubMed
  29. Safaiyan S, et al. 2016. Nat Neurosci. 10.1038/nn.4325. PubMed
  30. Gouna G, et al. 2021. J Exp Med. 218:. PubMed
  31. Roth JD, et al. 2018. World J Gastroenterol. 24:195. PubMed
  32. Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed
  33. Ribieras AJ, et al. 2022. Front Cardiovasc Med. 9:929466. PubMed
  34. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:H1932. PubMed
  35. Reales E, et al. 2015. J Cell Sci. 128:2661. PubMed
  36. Hagemann CA, et al. 2022. PLoS One. 17:e0275901. PubMed
  37. Jacob N, et al. 2011. J Immunol. 186:4984. PubMed
  38. Zhang M, et al. 2022. Front Oncol. 12:828041. PubMed
  39. Lorenz G, et al. 2022. J Innate Immun. :1. PubMed
  40. Seguin L, et al. 2017. Cancer Discov. 7:1464. PubMed
  41. Jia J, et al. 2020. Mol Cell. 951:77. PubMed
  42. Eich C, et al. 2016. PLoS One. 11: 0149637. PubMed
  43. Chao C, et al. 2012. Clin Cancer Res. 18:4702. PubMed
  44. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  45. Tarighat SS, et al. 2021. Int J Mol Sci. 22:. PubMed
  46. Bjørnholm KD, et al. 2021. Basic Clin Pharmacol Toxicol. 128:103. PubMed
  47. Martinez L, et al. 2020. J Immunol. 205:2545. PubMed
RRID
AB_1134237 (BioLegend Cat. No. 125401)
AB_1134237 (BioLegend Cat. No. 125402)

Antigen Details

Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.

Gene ID
16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org
Go To Top Version: 6    Revision Date: 01/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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