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Purified anti-PSD95 Antibody (Previously Covance catalog# MMS-5182)

Pricing & Availability
Clone
K28/43 (See other available formats)
Regulatory Status
RUO
Other Names
Disks large homolog 4, discs large homolog 4, Tax interaction protein 15, synapse-associated protein 90, postsynaptic density protein 95, post-synaptic density protein 95, Dlgh4, PSD-95, PSD95, SAP90, SAP90A, PSD-95 alpha 2b, PSD-95 beta
Previously
Covance Catalog# MMS-5182
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_K28slash43_PURE_PSD95_Antibody_WB_051418
Western blot of purified anti-PSD95antibody (clone K28/43). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20µg of rat brain membrane lysate. The blot was incubated with 0.05 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_K28slash43_PURE_PSD95_Antibody_WB_051418
    Western blot of purified anti-PSD95antibody (clone K28/43). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20µg of rat brain membrane lysate. The blot was incubated with 0.05 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_K28slash43_PURE_PSD95_Antibody_2_IHCP_051418
    IHC staining of purified anti-PSD95 antibody (clone K28/43) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 3_K28slash43_PURE_PSD95_Antibody_IHCP_051418
    IHC staining of purified anti-PSD95 antibody (clone K28/43) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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810401 100 µL £113
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Description

PSD95, also known as SAP-90, is a member of the MAGUK family, encoded by the the DLG4 gene.  At postsynaptic sites, it mediates the scaffolding of NMDA receptors, AMPA receptors, potassium channels, and other associated signaling protein clusters. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested

IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), Immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P).

Product Citations
  1. Miyadera K, et al. 2022. Proc Natl Acad Sci U S A. 119:e2117038119. PubMed
  2. Sarn N, et al. 2020. Mol Psychiatry. . PubMed
  3. Shi Y, et al. 2020. J Alzheimers Dis. 76:1375. PubMed
  4. Brown EA, et al. 2018. Mol Autism. 9:48. PubMed
  5. Szabó LE, et al. 2021. Sci Rep. 1.413194444. PubMed
  6. Yamasaki S, et al. 2022. iScience. 25:103657. PubMed
  7. Inouye MO, et al. 2022. Life Sci Alliance. 5:. PubMed
  8. Ivanova D, et al. 2021. Science Advances. 7(18):. PubMed
  9. Lautz JD, et al. 2021. Cell Rep. 37:110076. PubMed
  10. Rosa J, et al. 2022. Cells. 11:. PubMed
  11. Stillman M, et al. 2022. Sci Rep. 12:3207. PubMed
  12. Comer AL, et al. 2020. PLoS Biol. 18:e3000604. PubMed
RRID
AB_2564750 (BioLegend Cat. No. 810401)

Antigen Details

Structure
Expected MW: 100 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
1742 View all products for this Gene ID
UniProt
View information about PSD95 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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