Alexa Fluor® 647 anti-CD49C (Integrin α3) Antibody, CD49C, P1B5

Pricing & Availability

Clone: P1B5 (See other available formats)
Regulatory Status: RUO
Other Names: GAP-B3, GAPB3, ILNEB, MSK18, VCA-2, VL3A, VLA3a, Integrin alpha-3, FRP-2, galactoprotein B3, VLA-3 subunit alpha, CD49 antigen-like family member C, antigen identified by monoclonal antibody J143, alpha-3 subunit
Isotype: Mouse IgG1, κ
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Product Citations: publications

P1B5_A647_CD49C_Antibody_051920 — IHC staining of Alexa Fluor® 647 anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. The slide was mounted with fluoromount G with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.

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865203

25 µg

123€

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865204

100 µg

259€

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Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: The P1B5 monoclonal antibody was generated against human HT1080 cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: This antibody recognizes the a3 subunit.
RRID: AB_2861093 (BioLegend Cat. No. 865203)
AB_2861093 (BioLegend Cat. No. 865204)

Antigen Details

Structure: CD49c is a 150 kD type I transmembrane glycoprotein.
Distribution: Cellular distribution: Plasma membrane.
Tissue distribution: Endothelial cells, epithelial cells, and dermal fibroblast.
Function: CD49c plays a role in cell-cell and cell-matrix adhesion.
Ligand/Receptor: Kalinin, collagen, laminin-1, laminin-5 and entactin, fibronectin
Cell Type: Endothelial cells, Epithelial cells, Fibroblasts
Biology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Gene ID: 3675 View all products for this Gene ID
UniProt: View information about CD49c on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Frozen Sections

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Other Formats

View All CD49C Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-CD49C (Integrin α3)	P1B5	IHC-F,FC,IP
Purified anti-CD49C (Integrin α3)	P1B5	IHC-F,ICC,FC,IP
Alexa Fluor® 647 anti-CD49C (Integrin α3)	P1B5	IHC-F

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/19/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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