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Brilliant Violet 510™ anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1.4_BV510_Ly-6C_Antibody_FC_072313
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 510™ (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
  • HK1.4_BV510_Ly-6C_Antibody_FC_072313
    C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 510™ (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
Compare all formats See Brilliant Violet 510™ spectral data
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128033 125 µL 141€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Wang W, et al. 2022. Dev Cell. 57:228. PubMed
  2. Lees-Shepard JB, et al. 2022. J Clin Invest. 132: . PubMed
  3. Marquardt RM, et al. 2022. Int J Mol Sci. 23: . PubMed
  4. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  5. Siret C, et al. 2022. Nat Commun. 13:7366. PubMed
  6. Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed
  7. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  8. Feng J, et al. 2022. Immunity. 55:405. PubMed
  9. Pfeifhofer-Obermair C, et al. 2022. Bio Protoc. 12:e4378. PubMed
  10. Pomatto-Watson LCD, et al. 2022. Geroscience. 44:2471. PubMed
  11. Bonifacio JPPL, et al. 2022. J Virol. 96:e0087122. PubMed
  12. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  13. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  14. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  15. Carvelli J, et al. 2020. Nature. 588:146. PubMed
  16. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  17. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  18. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  19. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  20. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
  21. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  22. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  23. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  24. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  25. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  26. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
  27. Lang V, et al. 2021. Elife. 10:. PubMed
  28. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  29. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  30. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  31. Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed
  32. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  33. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  34. Kullberg M, et al. 2015. Nanomedicine. 11: 1355-1363. PubMed
  35. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  36. Ledo JH, et al. 2020. PLoS One. e0237773:15. PubMed
  37. Mihalik NE, et al. 2021. AAPS PharmSciTech. 22:191. PubMed
  38. Sheikh AA, et al. 2019. Cell Rep. 28:1758. PubMed
  39. Gardner P, et al. 2017. Sci Rep. 7:40830. PubMed
  40. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  41. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  42. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  43. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  44. Saber M, et al. 2021. J Neurotrauma. 38:2862. PubMed
  45. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  46. Ledo JH, et al. 2020. Mol Psychiatry. . PubMed
  47. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  48. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
RRID
AB_2562351 (BioLegend Cat. No. 128033)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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