Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™) Antibody, Ly-6C, HK1.4

Pricing & Availability

Clone: HK1.4 (See other available formats)
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus C
Isotype: Rat IgG2c, κ
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285011

50 µg

269€

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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8⁺ T cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: L3 cloned CTL cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone HK1.4 does not block the binding of clone RB6-8C5⁸.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells^1-3 and immunohistochemistry of frozen sections⁴.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106278 (BioLegend Cat. No. 285011)

Antigen Details

Structure: 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution: Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8⁺ T cells.
Cell Type: Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.
Gene ID: 17067 View all products for this Gene ID
UniProt: View information about Ly-6C on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All Ly-6C Reagents Request Custom Conjugation

Description	Clone	Applications
Pacific Blue™ anti-mouse Ly-6C	HK1.4	FC
APC anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C	HK1.4	FC,IHC-F
Biotin anti-mouse Ly-6C	HK1.4	FC
FITC anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 647 anti-mouse Ly-6C	HK1.4	FC
PE anti-mouse Ly-6C	HK1.4	FC
PerCP/Cyanine5.5 anti-mouse Ly-6C	HK1.4	FC
PE/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 488 anti-mouse Ly-6C	HK1.4	FC
Alexa Fluor® 700 anti-mouse Ly-6C	HK1.4	FC
APC/Cyanine7 anti-mouse Ly-6C	HK1.4	FC
PerCP anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 570™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 421™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 510™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 605™ anti-mouse Ly-6C	HK1.4	FC
Brilliant Violet 711™ anti-mouse Ly-6C	HK1.4	FC
Purified anti-mouse Ly-6C (Maxpar® Ready)	HK1.4	FC,CyTOF®
Brilliant Violet 785™ anti-mouse Ly-6C	HK1.4	FC
PE/Dazzle™ 594 anti-mouse Ly-6C	HK1.4	FC
APC/Fire™ 750 anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-A0013 anti-mouse Ly-6C	HK1.4	PG
Brilliant Violet 650™ anti-mouse Ly-6C	HK1.4	FC
TotalSeq™-C0013 anti-mouse Ly-6C	HK1.4	PG
TotalSeq™-B0013 anti-mouse Ly-6C	HK1.4	PG
APC/Fire™ 810 anti-mouse Ly-6C Antibody	HK1.4	FC
Spark UV™ 387 anti-mouse Ly-6C	HK1.4	FC
PE/Fire™ 810 anti-mouse Ly-6C	HK1.4	FC
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™)	HK1.4	FC
PE/Cyanine5 anti-mouse Ly-6C	HK1.4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.