- Clone
- TS1/18 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V AS162
- Other Names
- Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
302115 | 100 µg | 190€ | ||||
302116 | 1 mg | 537€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Pig
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.
- Application References
-
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London.
- Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block)
- Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block)
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed
- Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
- Product Citations
-
- RRID
-
AB_2561483 (BioLegend Cat. No. 302115)
AB_2561483 (BioLegend Cat. No. 302116)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
- Distribution
-
Leukocytes
- Function
- Adhesion and signaling in hematopoietic system
- Ligand/Receptor
- ICAMs
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301. - Gene ID
- 3689 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD18 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD18 | TS1/18 | FC |
PE anti-human CD18 | TS1/18 | FC |
PE/Cyanine5 anti-human CD18 | TS1/18 | FC |
Purified anti-human CD18 | TS1/18 | FC,Block |
APC anti-human CD18 | TS1/18 | FC |
Ultra-LEAF™ Purified anti-human CD18 | TS1/18 | FC,Block |
PE/Cyanine7 anti-human CD18 | TS1/18 | FC |
PerCP/Cyanine5.5 anti-human CD18 | TS1/18 | FC |
PE/Dazzle™ 594 anti-human CD18 | TS1/18 | FC |
TotalSeq™-A0385 anti-human CD18 | TS1/18 | PG |
Alexa Fluor® 700 anti-human CD18 | TS1/18 | FC |
APC/Fire™ 750 anti-human CD18 | TS1/18 | FC |
TotalSeq™-C0385 anti-human CD18 | TS1/18 | PG |
TotalSeq™-B0385 anti-human CD18 | TS1/18 | PG |
APC/Cyanine7 anti-human CD18 | TS1/18 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-human CD18
Human peripheral blood lymphocytes stained with TS1/18 FITC -
PE anti-human CD18
Human peripheral blood lymphocytes stained with TS1/18 PE Pre-lysed human blood leukocytes were stained with CD18 (clo... -
PE/Cyanine5 anti-human CD18
Human peripheral blood lymphocytes stained with TS1/18 PE/Cy... -
Purified anti-human CD18
Human peripheral blood lymphocytes stained with purified TS1... -
APC anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
Ultra-LEAF™ Purified anti-human CD18
Human peripheral blood lymphocytes stained with purified TS1... -
PE/Cyanine7 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PerCP/Cyanine5.5 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PE/Dazzle™ 594 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
TotalSeq™-A0385 anti-human CD18
-
Alexa Fluor® 700 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
APC/Fire™ 750 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
TotalSeq™-C0385 anti-human CD18
-
TotalSeq™-B0385 anti-human CD18
-
APC/Cyanine7 anti-human CD18
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us