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Alexa Fluor® 647 anti-Histone H3.3 Phospho (Ser31) Antibody

Pricing & Availability
Clone
1A8G10 (See other available formats)
Regulatory Status
RUO
Other Names
Histone-H3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1A8G10_A647_Histone_H3point3_Phospho_Antibody_1_012422
HeLa cells were incubated with (left) or without (right) nocodozole overnight, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer then intracellularly stained with DAPI and Histone H3.3 Phospho (Ser31) (clone 1A8G10) Alexa Fluor® 647.
  • 1A8G10_A647_Histone_H3point3_Phospho_Antibody_1_012422
    HeLa cells were incubated with (left) or without (right) nocodozole overnight, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer then intracellularly stained with DAPI and Histone H3.3 Phospho (Ser31) (clone 1A8G10) Alexa Fluor® 647.
  • 1A8G10_A647_Histone_H3point3_Phospho_Antibody_2_012422
    HeLa cells were stimulated with (left) or without (right) nocodozole for 24 hours, fixed and permeabilized with cold 70% ethanol, then intracellularly stained with DAPI and Histone H3.3 Phospho (Ser31) (clone 1A8G10) Alexa Fluor® 647.
See Alexa Fluor® 647 spectral data
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687703 25 tests $206
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687704 100 tests $452
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Description

Histone 3, also known as H3, is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis. Phosphorylation of H3.3 Ser31 occurs during mitosis and is a chromatin mark associated with heterochromatin. This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Histone H3.3 S31ph peptide (24-37)(AARKSAP(phS)TGGVKK), Freund’s complete adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 1A8G10 detects histone H3.3 when phosphorylated at Ser31.

RRID
AB_2629552 (BioLegend Cat. No. 687703)
AB_2629552 (BioLegend Cat. No. 687704)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus.

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family
Phospho-Proteins
Antigen References

1. Chang FT, et al. 2015. Nucleic Acids Res. 43:2603.
2. Sharma K, et al. 2014. Cell Rep. 8:1583.
3. Yoshimi T, et al. 2013. Monoclon. Antib. Immunodiagn. Immunother. 32:119.

Gene ID
3020 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Histone
Specificity Alt (DOES NOT SHOW ON TDS):
Histone
App Abbreviation (DOES NOT SHOW ON TDS):
ICFC
UniProt
View information about Histone on UniProt.org
Go To Top Version: 3    Revision Date: 07/20/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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