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Alexa Fluor® 647 anti-human HLA-C Antibody

Pricing & Availability
Clone
DT-9 (See other available formats)
Regulatory Status
RUO
Other Names
HLAC, MHC Class I Antigen C
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
DT-9_A647_HLA-C_Antibody_091521.png
Human peripheral blood lymphocytes were stained with anti-human HLA-C (Clone DT-9) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • DT-9_A647_HLA-C_Antibody_091521.png
    Human peripheral blood lymphocytes were stained with anti-human HLA-C (Clone DT-9) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
373307 25 tests $188
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373308 100 tests $399
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Description

HLA-C belongs to the HLA class I heavy chain paralogues. MHC class I heavy chains associate with β2-microglobulin and are expressed on nearly all nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and thereby play an important role in cell-mediated immune responses and immune surveillance. Detailed characterization of the DT-9 clone indicates that it cross-reacts with HLA-E and certain HLA-A and HLA-B allotypes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified tamarin MHC class I molecules, corresponding to human HLA-C
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Corrah et al. 2011. J. Virol. 85: 3367.
  2. Apps et al. 2013. Science. 340:87.
  3. Braud et al. 1998. Curr. Biol. 8:1.
  4. Thomas R, et al. 2009. Nat. Genet. 41:1290. (HLA-A/B allotype cross-reactivity)
RRID
AB_2894582 (BioLegend Cat. No. 373307)
AB_2894582 (BioLegend Cat. No. 373308)

Antigen Details

Structure
Ig superfamily
Distribution

Nearly all cells

Function
Antigen peptide presentation
Ligand/Receptor
TCR, CD8
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3107 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
HLA-C
Specificity Alt (DOES NOT SHOW ON TDS):
HLA-C
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about HLA-C on UniProt.org
Go To Top Version: 1    Revision Date: 09/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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