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Alexa Fluor® 647 anti-mouse CD55 (DAF) Antibody

Pricing & Availability
Clone
RIKO-3 (See other available formats)
Regulatory Status
RUO
Other Names
Decay accelerating factor (DAF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
RIKO-3_A647_121508.jpg
C57BL/6 splenocytes stained with RA3-6B2 (B220) PE and Riko-3 Alexa Fluor® 647
  • RIKO-3_A647_121508.jpg
    C57BL/6 splenocytes stained with RA3-6B2 (B220) PE and Riko-3 Alexa Fluor® 647
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131805 25 µg $147
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131806 100 µg $347
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Description

CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isofrom and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Cao Q, et al. 2012. Am J Physiol Cell Physiol. 302:1731. PubMed.
Product Citations
  1. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  2. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  3. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  4. Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
  5. Inoue N, et al. 2020. Development. . PubMed
RRID
AB_1279261 (BioLegend Cat. No. 131805)
AB_1279261 (BioLegend Cat. No. 131806)

Antigen Details

Structure
60-70 kd single chain glycoprotein. While human CD55 contains only one CD55 gene, two distinct CD55 genes have been identified in the mouse, referred to as GPI-DAF and transmembrane(TM)-DAF. GPI-DAF is expressed only in testis.
Distribution

Red blood cells and most of the splenocytes, including B cells and T cells, and also many non-hematopoetic cells.

Function
Protect from complement attack by inhibition the deposition of C3 on antibody-sensitized cells.
Cell Type
B cells, Erythrocytes, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Miwa T et al. 2001. Immunology 104:207
2. Lin FY et al. 2001 Immunology 104:215

Gene ID
13136 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD55
Specificity Alt (DOES NOT SHOW ON TDS):
CD55
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD55 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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