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PE anti-human CD229 (Ly-9) Antibody

Pricing & Availability
Clone
HLy-9.1.25 (See other available formats)
Regulatory Status
RUO
Other Names
Ly9, Ly-9, SLAMF3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HLy-9dot1dot25_PE_011408
Human peripheral blood lymphocytes stained with HLy-9.1.25 PE
  • HLy-9dot1dot25_PE_011408
    Human peripheral blood lymphocytes stained with HLy-9.1.25 PE
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326107 25 tests $134
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326108 100 tests $292
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Description

CD229 is a 100-120 kD glycoprotein. It is a member of the SLAM family, a CD2 subset of the Ig superfamily, known as Ly9 or SLAMF3. CD229 is expressed on T cells, B cells, NK cells, and thymocytes. It functions as a homophilic adhesion molecule through binding to CD229 itself. The cytoplasmic tail of CD229 binds to SAP and Grb2 proteins. CD229 is involved in enhancing T cell activation and Th2 polarization. HLy9.1.25 antibody recognizes epitope A in the first Ig domain of Ly9 molecule.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
300.19 cell line transfected with the CD229 full-length DNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, and immunocytochemistry3. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 326109 & 326110).

Application References

(PubMed link indicates BioLegend citation)
  1. de la Fuente MA, et al. 2001. Blood 97:3513.
  2. Martin M, et al. 2005. J. Immunol. 174:5977.
  3. Romero X, et al. 2005. J. Immunol. 174:7033.
Product Citations
  1. Besseling PJ, et al. 2023. APL Bioeng. 7:026107. PubMed
RRID
AB_2137938 (BioLegend Cat. No. 326107)
AB_2137938 (BioLegend Cat. No. 326108)

Antigen Details

Structure
100-120 kD glycoprotein, SLAM family, CD2 subset of Ig superfamily
Distribution

T cells, B cells, NK cells, thymocytes

Function
Homophilic adhesion, T cell activation, Th2 cell polarization
Ligand/Receptor
CD229
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sandrin MS, et al. 1996. Immunogenetics 43:13.
2. de la Fuente MA, et al. 2001. Blood 97:3513.
3. Romero X, et al. 2005. J. Immunol. 174:7033.

Gene ID
4063 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD229
Specificity Alt (DOES NOT SHOW ON TDS):
CD229
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD229 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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