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PE anti-human CD325 (N-Cadherin) Antibody

Pricing & Availability
Clone
8C11 (See other available formats)
Regulatory Status
RUO
Other Names
Cadherin-2, Neural cadherin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8C11_PE_101310
Human acute myeloid leukemia cell line (KG1) were stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 8C11_PE_101310
    Human acute myeloid leukemia cell line (KG1) were stained with CD325 (clone 8C11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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350806 25 tests $129
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350805 100 tests $273
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Description

CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human N-cadherin extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The mAb 8C11 recognizes the amino acids 92–593 of CD325, located between the extracellular cadherin structural domain (EC) 3 and 4. Additional reported applications (for the relevant formats) include: immunofluorescence1,3,6, motility inhibition of N-cadherin-expressing cells2, and Western blot2,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF)
  2. Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB)
  3. Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF)
  4. Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB)
  5. Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC)
  6. Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF)
Product Citations
  1. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  2. Huber M, et al. 2023. Nat Commun. 14:2143. PubMed
  3. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  4. Sandquist E, et al. 2016. PLoS One. 11: 0156310. PubMed
  5. Cai X, et al. 2014. PLoS One. 9:108942. PubMed
  6. Zhang W, et al. 2015. PLoS One. 10:125995. PubMed
  7. Kasagi Y, et al. 2018. Cell Mol Gastroenterol Hepatol. 5:333. PubMed
RRID
AB_10660824 (BioLegend Cat. No. 350806)
AB_10660824 (BioLegend Cat. No. 350805)

Antigen Details

Structure
Member of cadherin family, Single pass transmembrane protein, extracellular region consist of five EC domains, one cytoplasmic domain, 130 kD
Distribution

Stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, some types of carcinoma cells

Function
Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility
Interaction
Catenins
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Colomiere M, et al. 2009. Brit. J. Cancer 100:134.
2. Yan W, et al. 2010. J. Biol. Chem. 285:14042.
3. Mosnier JF, et al. 2009. Mod. Pathol. 22:182.
4. Gao L, et al. 2010. Stem Cells 28:564.

Gene ID
1000 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD325
Specificity Alt (DOES NOT SHOW ON TDS):
CD325
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD325 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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