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PE anti-human Tim-4 Antibody

Pricing & Availability
Clone
9F4 (See other available formats)
Regulatory Status
RUO
Other Names
SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)
Isotype
Mouse IgG1, κ
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Product Citations
publications
9F4_PE_011712
Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 9F4_PE_011712
    Human Tim-4 transfected CHO cells were stained with TIM-4 (clone 9F4) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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354003 25 tests $153
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354004 100 tests $360
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Description

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, Tim-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The Tim-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to Tim-1-triggered Th2 responsiveness.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TIM-4- IgV Fc, fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 9F4 recognizes the FG loop of human Tim-41.

Application References

(PubMed link indicates BioLegend citation)
  1. Kobayashi N, et al. 2007. Immunity 27:927.
Product Citations
  1. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  2. Smith CM, et al. 2021. Biochem J. 478:3331. PubMed
  3. Yano H, et al. 2017. Med Mol Morphol. . 10.1007/s00795-017-0164-9. PubMed
RRID
AB_11124345 (BioLegend Cat. No. 354003)
AB_11124345 (BioLegend Cat. No. 354004)

Antigen Details

Structure
Type 1 transmembrane glycoprotein and mucin domain containing protein 4.
Distribution

Splenic CD14+, macrophages, immature DC.

Function
Tim-1 ligation to Tim-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Ligand/Receptor
Phosphatiylserine is the receptor of Tim-4, and Tim-4 is a ligand of Tim-1.
Cell Type
Dendritic cells, Macrophages
Biology Area
Immunology
Antigen References

1. Kobayashi N, et al. 2007. Immunity 27:927.
2. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.

Gene ID
91937 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Tim-4
Specificity Alt (DOES NOT SHOW ON TDS):
Tim-4
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about Tim-4 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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