Loading...

PerCP/Cyanine5.5 anti-human CD50 (ICAM-3) Antibody

Pricing & Availability
Clone
CBR-IC3/1 (See other available formats)
Regulatory Status
RUO
Other Names
ICAM-3
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
CBR-IC3slash1_PerCPCy55_CD50_Antibody_021918
Human peripheral blood lymphocytes were stained with anti-human CD50 (ICAM-3) (clone CBR-IC3/1) PerCP/Cyanine5.5 (closed histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • CBR-IC3slash1_PerCPCy55_CD50_Antibody_021918
    Human peripheral blood lymphocytes were stained with anti-human CD50 (ICAM-3) (clone CBR-IC3/1) PerCP/Cyanine5.5 (closed histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
330017 25 tests $176
Check Availability


Need larger quantities of this item?
Request Bulk Quote
330018 100 tests $382
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, αL/β2) integrin.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SKW3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications include: immunoprecipitation1 and blocking1-3.

Application References

(PubMed link indicates BioLegend citation)
  1. deFougerolles AR and Springer TA, et al. 1992. J. Exp. Med. 175:185. (IP, Block)
  2. Geijtenbeek T, et al. 2000. Cell 100:575. (Block)
  3. Giannoni E, et al. 2003. J Biol. Chem. 278:36763. (Block)
RRID
AB_2728279 (BioLegend Cat. No. 330017)
AB_2728279 (BioLegend Cat. No. 330018)

Antigen Details

Structure
Ig superfamily, type I membrane protein, 130 kD
Distribution

Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells

Function
Adhesion, costimulation
Ligand/Receptor
CD11a/CD18 (LFA-1), integrin αL/ß2
Cell Type
B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Valent P, et al. 2001. Immunol. Rev. 179:74.

Gene ID
3385 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD50
Specificity Alt (DOES NOT SHOW ON TDS):
CD50
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD50 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 02/19/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account