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PerCP/Cyanine5.5 anti-human CD89 Antibody

Pricing & Availability
Clone
A59 (See other available formats)
Regulatory Status
RUO
Workshop
V MR30
Other Names
FcαR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A59_PerCPCy5.5_CD89_Antibody_FC_021615
Human peripheral blood granulocytes were stained with CD89 (clone A59) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • A59_PerCPCy5.5_CD89_Antibody_FC_021615
    Human peripheral blood granulocytes were stained with CD89 (clone A59) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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354109 25 tests $176
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354110 100 tests $382
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Description

CD89, also known as FcαR, is a 55-100 kD glycosylated protein.  It belongs to the immunoglobulin gene family.  It is expressed on granulocytes, monocytes, and macrophages but is absent on T cells.  It can interact with IgA aggregates and plays an important role in IgA mediated immune responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ag8.653 myeloma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References

(PubMed link indicates BioLegend citation)
  1. Monteiro RC, et al. 1992. J. Immunol. 148:1764.
  2. Shen L. 1992. J. Leukoc. Biol. 51:373.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Rogers KA, et al. 2004. Immunology 113:178.
RRID
AB_2565474 (BioLegend Cat. No. 354109)
AB_2565474 (BioLegend Cat. No. 354110)

Antigen Details

Structure
Member of the immunoglobulin gene family and is a transmembrane glycoprotein, 55-100 kD
Distribution

Granulocytes, neutrophils, eosinophils, monocytes, macrophages

Function
Interacts with IgA opsonized targets and induces immunological processes such as phagocytosis, ADCC, and release of inflammatory mediators
Ligand/Receptor
IgA (monomeric, dimeric, polymeric and secretory IgA)
Cell Type
Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Patry C, et al. 1996. J. Immunol. 156:4442.
2. de Wit, et al. 1995. J. Immunol. 155:1203.
3. Honorio-França AC, et al. 2001. J. Leukoc. Biol. 69:289.

Gene ID
2204 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD89
Specificity Alt (DOES NOT SHOW ON TDS):
CD89
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD89 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 02/16/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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