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Purified anti-β-actin Antibody

Pricing & Availability
Clone
W16197A (See other available formats)
Regulatory Status
RUO
Other Names
ACTB, Actin, cytoplasmic 1, Beta-actin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16197A_PURE_beta-actin_Anitbody_1_101217
Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain and zebra fish were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 diluted (0.1 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • W16197A_PURE_beta-actin_Anitbody_1_101217
    Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain and zebra fish were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 diluted (0.1 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • W16197A_PURE_beta-actin_Anitbody_2_101217
    Total lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:800 diluted (0.25 µg/mL), 1:1600 diluted (0.125 µg/mL), 1:3200 diluted (0.0625 µg/mL), 1:6400 diluted (0.03125 µg/mL) and 1:12800 diluted (0.0155 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • W16197A_PURE_beta-actin_Anitbody_3_081619
    Western blot of purified anti-β-actin antibody (clone W16197A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
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664801 25 µg $101
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664802 100 µg $253
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, Zebrafish, Drosophila
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human ACTB peptide (1-13 a.a.).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg/ml in human, mouse, rat and zebrafish and 0.1 - 0.5 µg/ml in Drosophila. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

Product Citations
  1. Wang L, et al. 2022. Viruses. 14: . PubMed
  2. Park SJ, et al. 2023. Int J Mol Sci. 24: . PubMed
  3. Lippert TP, et al. 2021. Nat Commun. 0.855555556. PubMed
  4. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  5. Balcioglu O, et al. 2020. Breast Cancer Res. 1.003472222. PubMed
  6. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
RRID
AB_2721350 (BioLegend Cat. No. 664801)
AB_2721350 (BioLegend Cat. No. 664802)

Antigen Details

Structure
375 amino acids with a predicted molecular weight of 42 kD.
Distribution

Cytoplasm

Function
Actins are highly conserved proteins that are involved in cell motility.
Interaction
Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
60 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
beta-actin
Specificity Alt (DOES NOT SHOW ON TDS):
β-actin
App Abbreviation (DOES NOT SHOW ON TDS):
WB
UniProt
View information about beta-actin on UniProt.org
Go To Top Version: 2    Revision Date: 08/16/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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