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Purified anti-H2A.X Phospho (Ser139) Antibody

Pricing & Availability
Clone
2F3 (See other available formats)
Regulatory Status
RUO
Other Names
H2A.x, H2a/x, Histone 2A, Histone 2A.X, Gamma-H2AX
Isotype
Mouse IgG1, κ
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Product Citations
publications
a-2F3_PURE_H2AdotX_Antibody_WB_122117
Total lysates (15 µg protein) from untreated HeLa (Lane 1) and UV treated HeLa (Lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL Purified anti-H2A.X Phospho (Ser139) antibody, clone 2F3 (upper) or 1:2000 diluted anti-GAPDH antibody, clone 1D4 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for both anti-H2A.X Phospho (Ser139) antibody and anti-GAPDH antibody. Lane M: Molecular weight ladder
  • a-2F3_PURE_H2AdotX_Antibody_WB_122117
    Total lysates (15 µg protein) from untreated HeLa (Lane 1) and UV treated HeLa (Lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL Purified anti-H2A.X Phospho (Ser139) antibody, clone 2F3 (upper) or 1:2000 diluted anti-GAPDH antibody, clone 1D4 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for both anti-H2A.X Phospho (Ser139) antibody and anti-GAPDH antibody. Lane M: Molecular weight ladder
  • b-2F3_PURE_H2AdotX_Antibody_ICC_051019
    HeLa cells were stained with purified anti-H2A.X Phospho (Ser 139) (clone 2F3) antibody, followed by staining with DyLightTM 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).
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613401 25 µg $118
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Description

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for DNA repair and maintaining genomic stability and for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified peptide
Formulation
This H2A.X antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunohistochemistry on paraffin embedded sections2, immunofluorescence microscopy3-9, Western blotting 10-12, and flow cytometry1,13. Clone 2F3 cross-reacts with mouse4.

Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry

1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C.
2. Prepare cells of interest.
3. Wash 1X with PBS, centrifuge at 350g for 5 min.
4. Discard the supernatant and vortex to loosen cell pellet.
5. Add pre-cooled 70% ethanol drop by drop, while vortexing.
6. Incubate at -20°C for 60 minutes.
7. Wash 3X with BioLegend Cell Staining Buffer and resuspend the cells at 0.5-1 X 107 cells/ml in the cell staining buffer.
8. Perform immunofluorescent staining for flow cytometry.

Application References

(PubMed link indicates BioLegend citation)
  1. Jha JC, et al. 2013. J. Virol. 87:5255. (FC) PubMed
  2. Akbay A, et al. 2008. Am J Pathol. 173:536. (IHC) PubMed
  3. Mochizuki K, et al.2008.J cell Sci.121:2148. (IF) PubMed
  4. Xiao R, et al. 2007. Mol Cell Biol.27:5393. (IF) PubMed
  5. Rossi DJ, et al. 2007. Nature. 447:725. (IF) PubMed
  6. Loidl J, et al. 2009. Mol Cell Biol. 20:2048. (IF) PubMed
  7. Beels L, et al. 2009. Circulation. 120:1903. (IF) PubMed
  8. Suzuki K, et al. 2010. Nucleic Acids Res. 38:e129. (IF) PubMed
  9. Lukaszewicz A. 2010. Chromasoma Apr 27. [Epub ahead of print] (IF) PubMed
  10. Yamada C, et al. 2010 J. Biol. Chem. 285:16693. (WB) PubMed
  11. Bu Y, et al. 2010, Biochem Biophys Res Commun. 397:157. (WB) PubMed
  12. Massignan T, et al. 2010. J. Biol Chem. 285:7752. (WB) PubMed
  13. Banath JP, et al. 2010. BMC Cancer 10:4 (FC)
  14. Zhang M., et al. 2011. Cancer Res. 23:7155. PubMed
  15. Kuefner MA, et al. 2012. Radiology 264:59. PubMed
  16. Yoshihara Y, et al. 2012. Biochem Biophys Res Cmmun. 421:57. PubMed
  17. Titus S, et al. 2013. Sci Transl Med. 13:21. PubMed
  18. Crown KN, et al. 2013. G3. 6:1927. PubMed
  19. Schenkwein D, et al. 2013. Nucleic Acids Res. 41:e61. PubMed
  20. Zhadanova NS, et al. 2014. Mol Cell Biol. 34:2786. PubMed
  21. Horrell SA, et al. 2014. Eukaryot Cell. 13:1300. PubMed
  22. Maya-Mendoza A, et al. 2015. Mol Oncol. 9:601. PubMed
Product Citations
  1. Li M, et al. 2011. Mol Cell Biol. 31:2090. PubMed
  2. Kuefner M, et al. 2012. Radiology. 264:59:00. PubMed
  3. Xu C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:327. PubMed
  4. Hsiao HW, et al. 2023. Biomolecules. 13: . PubMed
  5. Mendoza-Munoz PL, et al. 2023. NAR Cancer. 5:zcad003. PubMed
  6. Oelschläger L, et al. 2023. Cells. 12: . PubMed
  7. Kulbay M, et al. 2022. Mol Cell Biochem. 477:2213. PubMed
  8. Chomiak AA, et al. 2022. iScience. 25:104354. PubMed
  9. Marchena-Cruz E, et al. 2023. Cell Rep. 42:112148. PubMed
  10. Roggan MD, et al. 2023. Front Public Health. 11:1063250. PubMed
  11. Saez-Ayala M, et al. 2023. Nat Commun. 14:3079. PubMed
  12. Feng W, et al. 2017. Proc Natl Acad Sci U S A. 114(2):406-411. PubMed
  13. Nyeste A, et al. 2016. J Biol Chem. 291: 4473 - 4486. PubMed
  14. Jha H, et al. 2013. J Virol. 87:5255. PubMed
  15. Xiao R, et al. 2007. Mol Cell Biol. 27:5393. PubMed
  16. Teloni F et al. 2019. Mol Cell. 73(4):670-683 . PubMed
  17. Wang C, et al. 2020. DNA Repair (Amst). 95:102946. PubMed
  18. Nomura D, et al. 2021. Biol Pharm Bull. 44:642. PubMed
  19. Zhong B, et al. 2019. Am J Cancer Res. 9:79. PubMed
  20. Wang R, et al. 2020. Proc Natl Acad Sci U S A. 117:33436. PubMed
  21. Suzuki T, et al. 2021. Nucleic Acids Res. 49:e40. PubMed
  22. Roeschert I, et al. 2021. Nat Cancer. 2:312. PubMed
  23. Yamauchi M, et al. 2017. Sci Rep. 7:41812. PubMed
  24. Kaul Z, et al. 2011. EMBO Rep. 13:52. PubMed
  25. Taglialatela A et al. 2017. Molecular cell. 68(2):414-430 . PubMed
  26. Murray-Nerger LA, et al. 2021. Nucleic Acids Res. 49:2044. PubMed
  27. Stoyas CA, et al. 2020. Neuron. 630:105. PubMed
  28. Maffucci P, et al. 2018. J Clin Invest. 128:5489. PubMed
  29. Hopp AK, et al. 2021. Mol Cell. 81:340. PubMed
  30. Gatti M, et al. 2020. Cell Rep. 32:107985. PubMed
  31. Kohutova A, et al. 2019. FASEB J. :fj201801877RR. PubMed
  32. Yoshihara Y, et al. 2012. Biochem Biophys Res Commun. 421:57. PubMed
  33. Maya-Mendoza A, et al. 2015. Mol Oncol. 9:601. PubMed
  34. Pedersen R, et al. 2016. Nat Commun. 7:13887. PubMed
  35. Massonneau J, et al. 2018. FEBS Open Bio. 8:416. PubMed
  36. Kwasna D et al. 2018. Molecular cell. 70(1):150-164 . PubMed
  37. Mlcochova P, et al. 2020. Cell Rep. 30:3972. PubMed
  38. Michelena J, et al. 2021. Life Sci Alliance. 4: . PubMed
  39. Pelicci S, et al. 2022. Nanomaterials (Basel). 12. PubMed
  40. Schneider MWG, et al. 2022. Nature. 609:183. PubMed
  41. Gavande NS, et al. 2020. Nucleic Acids Res. 48:11536. PubMed
  42. Chalker S 2014. Eukaryot Cell. 13:1300. PubMed
  43. Friedman J, et al. 2018. J Immunother Cancer. 6:59. PubMed
  44. Nguyen TM, et al. 2020. Nucleic Acids Res. 48:2621. PubMed
  45. Michelena J, et al. 2018. Nat Commun. 9:2028. PubMed
  46. Guo Y, et al. 2022. iScience. 25:104519. PubMed
  47. Lee S, et al. 2021. Nat Commun. 12:6309. PubMed
  48. Jelinkova S, et al. 2021. Int J Mol Sci. 22: . PubMed
  49. Qin Y, et al. 2019. Mol Oncol. 13:1419. PubMed
  50. Wang Z, et al. 2019. J Biol Chem. 294:3909. PubMed
  51. Mochizuki K, et al. 2008. J Cell Sci. 121:2148. PubMed
  52. Mochizuki J 2009. Mol Biol Cell. 2.519444444. PubMed
  53. Martinelli P, et al. 2011. Blood. 117:6617. PubMed
  54. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  55. Cuella-Martin R, et al. 2021. Cell. 184(4):1081-1097.e19. PubMed
  56. Michelena J, et al. 2019. J Cell Biol. 218:2865. PubMed
  57. Lezaja A, et al. 2021. Nat Commun. 12:3827. PubMed
  58. Oshima K, et al. 2020. Nat Cancer. 1:1113. PubMed
  59. Callender LA, et al. 2021. Nat Commun. 12:3379. PubMed
  60. Titus S, et al. 2013. Sci Transl Med. 13:21. PubMed
  61. Delaney JR, et al. 2020. PLoS Genet. 16:e1008558. PubMed
  62. Saxena S et al. 2018. Cell reports. 25(12):3273-3282 . PubMed
  63. Sang M, et al. 2018. Oncol Rep. 39:2749. PubMed
  64. Ueno H, et al. 2022. Commun Biol. 5:571. PubMed
  65. Paculova H, et al. 2017. Tumour Biol. 39:1010428317727479. PubMed
  66. Hjorth–Jensen K, et al. 2018. Nucleic Acids Res. 46:9484. PubMed
  67. Mlcochova P,et al. 2017. EMBO J.. 10.15252/embj.201796880. PubMed
  68. Furia L, et al. 2022. Front Oncol. 12:960734. PubMed
  69. Switonski PM, et al. 2021. Cell Rep. 37:110062. PubMed
  70. Kychygina A, et al. 2021. Sci Rep. 11:13195. PubMed
  71. Kim C, et al. 2021. Nat Commun. 12:1097. PubMed
  72. Kim IK, et al. 2020. Cell Death Differ. 27:2263. PubMed
  73. Sakata R, et al. 2021. Cell Reports. 35(4):108999. PubMed
  74. Stobezki R, et al. 2020. Reprod Sci. 27:940. PubMed
  75. Udugama M, et al. 2015. Nucleic Acids Res. 43: 10227 - 10237. PubMed
  76. Sandoval P, et al. 2015. PLoS Genet. 11: 1005405. PubMed
  77. Pellegrino S, et al. 2017. Cell Reports. 10.1016/j.celrep.2017.05.016. PubMed
  78. Furia L, et al. 2022. Int J Mol Sci. 23:. PubMed
  79. Perucca P et al. 2018. Biochimica et biophysica acta. 1865(6):898-907 . PubMed
  80. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  81. Garg J, et al. 2020. Current Biology. 29(14):2371-2379. PubMed
  82. Przetocka S, et al. 2018. Mol Cell. 72:568. PubMed
  83. Akbay E, et al. 2008. Am J Pathol. 173:536. PubMed
  84. Crown K, et al. 2013. g3. 3:1927. PubMed
  85. Zhdanova N, et al. 2014. Mol Cell Biol. 34:2786. PubMed
  86. Swift ML, et al. 2021. Cell Reports. 34(11):108840. PubMed
  87. Valianatos G,et al. 2017. PLoS One. 10.1371/journal.pone.0185801. PubMed
  88. Akematsu T et al. 2017. eLife. 6 pii: e26176. PubMed
  89. Massignan T, et al. 2010. J Biol Chem. 285:7752. PubMed
  90. Widodo N, et al. 2010. PLoS One. 5:e13536. PubMed
  91. Hirano J 2011. J Cell Biol. 192:263. PubMed
  92. Suzuki K, et al. 2010. Nucleic Acids Res. 38:e129. PubMed
  93. Xu Y, et al. 2020. Oncol Rep. 44:1455. PubMed
  94. Prendergast L, et al. 2020. Nat Commun. 3.606944444. PubMed
  95. Yamauchi S, et al. 2017. BMC Cancer. 10.1186/s12885-017-3621-x. PubMed
  96. Ogata T, et al. 2017. PLoS One. 10.1371/journal.pone.0179884. PubMed
  97. Amrenova A, et al. 2021. PLoS One. 16:e0249059. PubMed
RRID
AB_315795 (BioLegend Cat. No. 613401)
AB_315795 (BioLegend Cat. No. 613402)

Antigen Details

Structure
Basal histone, H2 histone family; 14 kD
Distribution

Nuclear

Function
Phosphorylated H2AX promotes DNA repair and maintains genomic stability. Important for recombination between immunoglobulin switch regions
Modification
Phosphorylation on Ser139 after double-stranded DNA breaks
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Neuroscience
Molecular Family
Phospho-Proteins
Antigen References

1. Mannironi C, et al.1989. Nucleic Acids Res. 17:9113.
2. Celeste A, et al. 2002. Science 296:922.
3. Bassing CH, et al. 2002. Proc. Natl. Acad. Sci. USA 99:8173.
4. Reina-San-Martin B, et al. 2003. J. Exp. Med. 197:1767.

Regulation
Synthesized in G1 and S-phase of cell cycle
Gene ID
3014 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
H2A.X Phospho Ser139
Specificity Alt (DOES NOT SHOW ON TDS):
H2A.X Phospho (Ser139)
App Abbreviation (DOES NOT SHOW ON TDS):
WB,ICC,ICFC
UniProt
View information about H2A.X Phospho Ser139 on UniProt.org

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Go To Top Version: 4    Revision Date: 11/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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