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Purified anti-human IgE Antibody

Pricing & Availability
Clone
MHE-18 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin E
Isotype
Mouse IgG1, κ
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Product Citations
publications
MHE-18_Pure_IgE_Antibody_061918
Overnight cultured human peripheral blood mononuclear cells stained with CD193/CCR3 (clone 5E8) APC and human IgE (clone MHE-18) Purified (left) or mouse IgG1, κ isotype control Purified (Right), followed by anti-mouse IgG1 PE (gated on lymphocyte population).
  • MHE-18_Pure_IgE_Antibody_061918
    Overnight cultured human peripheral blood mononuclear cells stained with CD193/CCR3 (clone 5E8) APC and human IgE (clone MHE-18) Purified (left) or mouse IgG1, κ isotype control Purified (Right), followed by anti-mouse IgG1 PE (gated on lymphocyte population).
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325502 100 µg $112
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Description

IgE is the seventh immunoglobulin made by B cells in the immune response.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.
Product Citations
  1. Balemans D,et al. 2017. Sci Rep. . 10.1038/s41598-017-12618-7. PubMed
  2. Maehara T, et al. 2019. Medicine (Baltimore). 98:e18300. PubMed
  3. Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed
  4. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  5. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  6. Cildag S, et al. 2021. Med Pharm Rep. 94:53. PubMed
RRID
AB_830847 (BioLegend Cat. No. 325502)

Antigen Details

Distribution

B cells

Cell Type
B cells
Biology Area
Immunology
Gene ID
3497 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IgE
Specificity Alt (DOES NOT SHOW ON TDS):
IgE
App Abbreviation (DOES NOT SHOW ON TDS):
FC,ELISA,IHC
UniProt
View information about IgE on UniProt.org
Go To Top Version: 2    Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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