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Purified anti-Vimentin Antibody

Pricing & Availability
Clone
W16220A (See other available formats)
Regulatory Status
RUO
Other Names
Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16220A_PURE_Vimentin_Antibody_1_091417
Total cell lysate (15 µg protein) from A20 (negative control), EL4, NIH3T3 and HeLa cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1: 500 (1 µg/mL) diluted purified anti-Vimentin antibody (clone W16220A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16220A_PURE_Vimentin_Antibody_1_091417
    Total cell lysate (15 µg protein) from A20 (negative control), EL4, NIH3T3 and HeLa cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1: 500 (1 µg/mL) diluted purified anti-Vimentin antibody (clone W16220A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16220A_PURE_Vimentin_Antibody_2_031819
    NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:1000 (0.5 µg/mL) anti-Vimentin (clone W16220A) overnight at 4 degrees followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG incubation for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. For negative control and titration data, refer to the supplemental data.
  • W16220A_PURE_Vimentin_Antibody_3_092523
    A20 cells (negative control, open histogram) or EL-4 cells (positive control, filled histogram) were fixed and permeabilized using True-Phos™ Perm buffer (Cat. No. 425401) and intracellularly stained with purified anti-Vimentin (clone W16220A), or purified Rat IgG2a, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400501), followed by Alexa Fluor™ 647 goat anti-Rat IgG (Cat. No. 405416).
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699301 25 µg $101
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699302 100 µg $253
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Description

Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5 and rheumatoid arthritis6, and also found after transplantation7.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1: 500-1:5000 diluted (0.1 - 1 µg/mL). For immunocytochemistry, the suggested use of this reagent is 1:250-1:2500 diluted (0.2 - 2 µg/mL). For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody does not cross-react with human (in-house tested).

May be weakly reactive with human Vimentin by WB.

Product Citations
  1. Liu M, et al. 2023. Sci Transl Med. eabm5663:15. PubMed
  2. Park J, et al. 2023. Front Pharmacol. 1275749:14. PubMed
  3. Heinrich A, et al. 2021. Cell Rep. 37:109885. PubMed
  4. Heinrich A, et al. 2020. Cell Rep. 31:107513. PubMed
RRID
AB_2716136 (BioLegend Cat. No. 699301)
AB_2716136 (BioLegend Cat. No. 699302)

Antigen Details

Structure
466 amino acids with a predicted molecular weight of 54 kD.
Distribution

Cytoplasm.

Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Interaction
HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1.
2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345.
3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429.
4. Scanlon CS, et al. 2013. J Dent Res. 92:114.
5. Thebault S, et al. 2002. J Immunol. 169:4046.
6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142.
7. Rose ML. 2013. Hum Immunol. 74:1459.

Gene ID
22352 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Vimentin
Specificity Alt (DOES NOT SHOW ON TDS):
Vimentin
App Abbreviation (DOES NOT SHOW ON TDS):
WB,ICC,ICFC
UniProt
View information about Vimentin on UniProt.org
Go To Top Version: 2    Revision Date: 09/25/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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