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Purified Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-PURE, SAV-PU, SAV
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Product Citations
publications
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405150 1 mg $71
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405151 10 mg $411
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Description

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical data sheet

Product Details

Formulation
Each vial contains Streptavidin lyophilized in sodium chloride buffer.
Preparation
Reconstitute the content of the vial in distilled water to the desired concentration.
Storage & Handling
For long-term storage, store the lyophilized streptavidin at -20°C or -70°C. After reconstitution, store the solution between 2°C and 8°C for up to 2 weeks, or store in aliquots at -20°C for up to 6 months.
Application

FC - Quality tested
ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed
  2. Urlaub D, et al. 2020. Curr Protoc Immunol. 131:e111. PubMed
  3. Ridge LA, et al. 2021. Cell Rep. 36:109610. PubMed

Antigen Details

Structure
52.8 kD tetrameric protein
Distribution

Streptavidin binds to biotin with high affinity

Ligand/Receptor
Biotin
Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
Biotin
Specificity Alt (DOES NOT SHOW ON TDS):
Biotin
App Abbreviation (DOES NOT SHOW ON TDS):
FC,ELISA Capture,ELISA Detection,ICC,IHC-F,IHC-P,IP,WB
UniProt
View information about Biotin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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