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Alexa Fluor® 488 anti-human CD282 (TLR2) Antibody

Pricing & Availability
Clone
TL2.1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Toll like receptor 2, TIL4, CD282
Isotype
Mouse IgG2a, κ
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Product Citations
publications
TL2dot1_Alx488_072307
Human peripheral blood monocytes stained with TL2.1 Alexa Fluor® 488
  • TL2dot1_Alx488_072307
    Human peripheral blood monocytes stained with TL2.1 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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309712 100 tests $293
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Description

Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR2-transfected CHO cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation. Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production1,3,7, immunoprecipitation1, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections2 and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections4 , and Western blotting2. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 309716) (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered).

Application References

(PubMed link indicates BioLegend citation)
  1. Flo T, et al. 2000. J. Immunol. 164:2064.
  2. Faure E, et al. 2001. J. Immunol. 166:2018.
  3. Sugawara S, et al. 2001. Infect. Immun. 69:4951.
  4. Droemann D, et al. 2003. Histochem. Cell Biol. 119:103.
  5. Chavakis E, et al. 2007. Circ. Res. 100:204.PubMed
  6. Fiala M, et al. 2007. Proc. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
  7. Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.PubMed
  8. Weiss DJ,et al. 2008.J. Leukoc. Biol. 83:48. PubMed
  9. Harris, KM., et al. 2011. J. Leukoc Biol. 90:727. PubMed.
  10. Elass-Rocahrd E, et al. 2012. J Biol Chem. 287:34432. PubMed.
Product Citations
  1. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
RRID
AB_2204582 (BioLegend Cat. No. 309712)

Antigen Details

Structure
IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
Distribution

Monocytes, granulocytes, endothelial and epithelial cells

Function
Activates innate host defense against bacterial pathogens
Ligand/Receptor
Microbial lipoproteins, peptidoglycans, lipoteichoic acid
Cell Type
Endothelial cells, Epithelial cells, Granulocytes, Monocytes, Neutrophils, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Lien E, et al. 1999. J. Biol. Chem. 274:33419.
2. Lien E, et al. 2001. J. Biol. Chem. 276:1873.
3. Sabroe I, et al. 2002. J. Immunol. 168:4701

Gene ID
7097 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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