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Alexa Fluor® 488 anti-human Sialyl Lewis X (dimeric) Antibody

Pricing & Availability
Clone
FH6 (See other available formats)
Regulatory Status
RUO
Other Names
CD15s, Stage-Specific Embryonic Antigen 1, SSEA-1, Sialyl Lewis X, sLex, sLe(x), Sialyl demeric LeX, Sialyl dermic Lewis X, Sialyl Lewis X-i, sLeX-i
Isotype
Mouse IgM, κ
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Product Citations
publications
FH6_AF488_SialylLewisX_Antibody_1_FC_081915
Human peripheral blood granulocytes were stained with sialyl lewis X (dimeric) (clone FH6) Alexa Fluor® 488 (filled histogram) or mouse IgM, κ Alexa Fluor® 488 isotype control (open histogram).
  • FH6_AF488_SialylLewisX_Antibody_1_FC_081915
    Human peripheral blood granulocytes were stained with sialyl lewis X (dimeric) (clone FH6) Alexa Fluor® 488 (filled histogram) or mouse IgM, κ Alexa Fluor® 488 isotype control (open histogram).
  • FH6_AF488_SialylLewisX_Antibody_2_FC_081915
    Human peripheral blood lymphocytes were stained with sialyl lewis X (dimeric) (clone FH6) Alexa Fluor® 488 (filled histogram) or mouse IgM, κ Alexa Fluor® 488 isotype control (open histogram).
Compare all formats See Alexa Fluor® 488 spectral data
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368105 25 tests $159
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Description

The FH6 antibody recognizes Sialyl Lewis X (demeric) on glycolipids or glycoproteins. It also recognizes Sialyl Lewis X with long carbohydrate attachments (Sialyl Lewis X-i). These antigens are expressed on human granulocytes, monocytes, small subsets of lymphocytes, some fetal tissues such as the fetal stomach, fetal colon, and fetal intestine, and a variety of cancer tissues. It is believed that these antigens are involved in cell adhesion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified 6B fucoganglioside absorbed to Salmonella minnesota.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511.
  2. Fukushi Y, et al. 1985. Cancer Res. 8:3711.
  3. Kannagi R, et al. 1986. Cancer Research 5:2619.
  4. Kobayashi M, et al. 2010. Anticancer Res. 30:593.
RRID
AB_2566072 (BioLegend Cat. No. 368105)

Antigen Details

Structure
Carbohydrate epitope; VI3NeuAcV3III3Fuc2nLc6.
Distribution

Granulocytes, monocytes, subpopulations of lymphocytes, fetal stomach, fetal colon, fetal small intestine, and a variety of cancer tissues.

Function
Cell adhesion.
Ligand/Receptor
Selectins.
Cell Type
Embryonic Stem Cells, Granulocytes, Lymphocytes, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511.
2. Kannagi R, et al. 1986. Cancer Research 5:2619.
3. Nakasaki H, et al. 1989. Cancer Research 49:3662.
4. Dohi T, et al. 1993. Anticancer Res. 13:1277.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about Sialyl Lewis X on UniProt.org
Go To Top Version: 1    Revision Date: 08/21/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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