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Alexa Fluor® 488 anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
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Product Citations
publications
A_GK1.5_AF488_071805
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) Alexa Fluor® 488 (solid line) or rat IgG2b, κ Alexa Fluor® 488 isotype control (broken line).
  • A_GK1.5_AF488_071805
    C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) Alexa Fluor® 488 (solid line) or rat IgG2b, κ Alexa Fluor® 488 isotype control (broken line).
  • B_GK1.5_A488_CD4_IHCF_040318
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594 (blue), 5 µg/ml of CD8 (clone 53-6.7) Alexa Fluor® 647 (red), and 5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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100425 25 µg $101
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100423 100 µg $229
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5-5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Liu X, et al. 2022. Chin Med J (Engl). 135:1821. PubMed
  2. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  3. Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed
  4. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  5. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
  6. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  7. Sun R, et al. 2023. PLoS One. 18:e0282722. PubMed
  8. Parlane N, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  9. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  10. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  11. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  12. Onishi S, et al. 2015. PLoS One. 10:126564. PubMed
  13. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  14. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  15. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  16. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  17. Inoue M, et al. 2014. Proc Natl Acad Sci U S A. 111:5295. PubMed
  18. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
  19. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  20. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  21. Min H, et al. 2015. PLoS One. 10: 0138201. PubMed
  22. Namai F, et al. 2020. Exp Mol Med. 52:1627. PubMed
  23. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  24. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  25. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  26. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  27. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  28. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  29. Collins N, et al. 2016. Nat Commun. 7:11514. PubMed
  30. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  31. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  32. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
  33. Bajic D, et al. 2020. Mol Cancer Ther. 19:2554. PubMed
  34. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  35. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  36. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  37. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  38. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  39. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  40. Park JS, et al. 2022. J Transl Med. 20:85. PubMed
RRID
AB_389302 (BioLegend Cat. No. 100425)
AB_389302 (BioLegend Cat. No. 100423)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC,IHC-F,ICC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
PE/Cyanine7 anti-mouse CD4 GK1.5 FC
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,ICC,SB
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Spark Blue™ 515 anti-mouse CD4 GK1.5 FC
Spark PLUS UV395™ anti-mouse CD4 GK1.5 FC
Spark Red™ 718 anti-mouse CD4 (Flexi-Fluor™) GK1.5 FC
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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