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Alexa Fluor® 488 anti-mouse DC Marker (33D1) Antibody

Pricing & Availability
Clone
33D1 (See other available formats)
Regulatory Status
RUO
Other Names
Dendritic Cell Marker, 33D1, DCIR2 (dendritic cell inhibitory receptor 2)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_33D1_A488_022309.jpg
C57BL/6 splenocytes stained with I-A/I-E (M5/114.15.2) APC and 33D1 Alexa Fluor® 488
  • A_33D1_A488_022309.jpg
    C57BL/6 splenocytes stained with I-A/I-E (M5/114.15.2) APC and 33D1 Alexa Fluor® 488
  • B_33D1_A488_022309.jpg
    C57BL/6 splenocytes stained with I-A/I-E (M5/115) APC and Alexa Fluor® 488 rat IgG2b isotype control
Compare all formats See Alexa Fluor® 488 spectral data
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124910 100 µg $265
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Description

33D1 antigen, also known as DCIR2 (dendritic cell inhibitory receptor 2), is reported to be a dendritic cell marker on subpopulation DC in the spleen, thymus, and Peyer's patch. It is reported that most N418 (CD11c)+ cells in spleen are also 33D1 positive. Bone marrow dendritic cells upregulate 33D1 antigen expression after incubation with GM-CSF.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3 and immunohistochemical staining of acetone-fixed frozen sections4.
 

Binding of clone 33D1 is calcium-dependent. We recommend staining in EDTA-free FACS buffer containing a source of Ca2+ ions.

Application References

(PubMed link indicates BioLegend citation)
  1. Crowley MT, et al. 1990. J. Immunol. Methods 133:55.
  2. Nussenzwig MC, et al. 1982. P. Natl. Acad. Sci. USA 79:161.
  3. Steinman RM, et al. 1983. J. Exp. Med. 157:613. (IF)
  4. Fischer HG, et al. 2000. J. Immunol. 164:4826. (IHC)
Product Citations
  1. Ding Z, Dahlin J 2016. Sci Rep. 6:28290. PubMed
RRID
AB_1186125 (BioLegend Cat. No. 124910)

Antigen Details

Structure
The antigen recognized by 33D1 is yet unknown.
Distribution

33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive.

Function
GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen.
Cell Type
Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about 33D1 on UniProt.org
Go To Top Version: 5    Revision Date: 01/26/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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