- Clone
- RME-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin E
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RME-1_AF488_IgE_Antibody_FC_1_041714 RME-1_AF488_IgE_Antibody_FC_1_041714](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RME-1_AF488_IgE_Antibody_FC_1_041714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
C57BL/6 mouse bone marrow cells were stained with CD45 PE, Ly-6G PerCP and IgE (clone RME-1) Alexa Fluor® 488 (top image) or rat IgG1, κ Alexa Fluor® 488 isotype control (bottom image). Data was gated on CD45 positive cells. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
406909 | 25 µg | $89 | ||||
406910 | 100 µg | $229 |
The RME-1 monoclonal antibody reacts with immunoglobulin E (IgE) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RME-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail, including IgE
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
This clone also recognizes IgE when bound to FceRI.
-
Application References
(PubMed link indicates BioLegend citation) -
- Jeong KT, et al. 2012. Int Immunopharmacol. 13:377. PubMed
- Product Citations
-
- RRID
-
AB_2563455 (BioLegend Cat. No. 406909)
AB_2563456 (BioLegend Cat. No. 406910)
Antigen Details
- Biology Area
- Immunology
- Gene ID
- 380792 View all products for this Gene ID
- UniProt
- View information about IgE on UniProt.org
Other Formats
View All IgE Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse IgE | RME-1 | ELISA,FC |
Purified anti-mouse IgE | RME-1 | ELISA,FC |
FITC anti-mouse IgE | RME-1 | FC |
PE anti-mouse IgE | RME-1 | FC |
Alexa Fluor® 488 anti-mouse IgE | RME-1 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-mouse IgE
-
Purified anti-mouse IgE
-
FITC anti-mouse IgE
IGEL cell line stained with RME-1 FITC -
PE anti-mouse IgE
IGEL-a2 cell line (IgE producing mouse hybridoma) stained wi... -
Alexa Fluor® 488 anti-mouse IgE
C57BL/6 mouse bone marrow cells were stained with CD45 PE, L...
Follow Us