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Alexa Fluor® 488 anti-mouse Perforin Antibody

Pricing & Availability
Clone
S16009B (See other available formats)
Regulatory Status
RUO
Other Names
Prf1, Pfp, Pfn, Pfn-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S16009B_A488_Perforin_Antibody_012424
C57BL/6 splenocytes were surface stained with anti-mouse CD49b (clone DX5) PE. Cells were then fixed, permeabilized, and then intracellularly stained with anti-mouse Perforin (clone S16009B) Alexa Fluor® 488 (left) or rat IgG2a, κ Alexa Fluor® 488 isotype control (right).
  • S16009B_A488_Perforin_Antibody_012424
    C57BL/6 splenocytes were surface stained with anti-mouse CD49b (clone DX5) PE. Cells were then fixed, permeabilized, and then intracellularly stained with anti-mouse Perforin (clone S16009B) Alexa Fluor® 488 (left) or rat IgG2a, κ Alexa Fluor® 488 isotype control (right).
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
154409 25 µg $159
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154410 100 µg $370
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse perforin transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone S16009B reacts with perforin from both C57BL/6 and BALB/c strains.

RRID
AB_3097554 (BioLegend Cat. No. 154409)
AB_3097554 (BioLegend Cat. No. 154410)

Antigen Details

Structure
70 kD Protein that oligomerizes for pore formation.
Distribution

CTL, NK (Cytoplasmic Granules)

Function
Target cell lysis
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Podack ER, et al, 1988, Immunol Rev, 103:203
  2. Konjar S, et al, 2010. Immunology, 131:257
  3. Trapani JA, et al, 2002, Nat Rev Immunol, 2:735
  4. Osinska I, et al. 2014, Cent Eur J Immnol, 39:109
Gene ID
18646 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/24/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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