Mouse TE-71 cell line was stained with anti-mouse podoplanin (clone PMab-1) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control (clone RTK2758) Alexa Fluor® 488 (open histogram).
Mouse TE-71 cell line was stained with anti-mouse podoplanin (clone PMab-1) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control (clone RTK2758) Alexa Fluor® 488 (open histogram).
C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10µg/ml of Alexa Fluor® 488 anti-mouse podoplanin (clone PMab-1, green), 10 µg/ml of Alexa Fluor® 594 anti-mouse CD326 (clone G8.8, red) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
Paraformaldehyde-fixed (4%), 500 μm-thick mouse kidney section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD31 Antibody (clone MEC13.3) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-Tubulin β 3 (TUBB3) Antibody (Clone TUJ1) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software. Watch the video.
The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
Interaction
Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
Ligand/Receptor
CLEC1B
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References
Farr A, et al. 1992. J Histochem Cytochem. 40:651.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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