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Alexa Fluor® 488 anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
UC7-13D5 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-γ/δ, γ/δ TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
UC7-13D5_Alx488_020708
C57BL/6 mouse splenocytes stained with CD3 (17A2) PE and UC7-13D5 Alexa Fluor® 488
  • UC7-13D5_Alx488_020708
    C57BL/6 mouse splenocytes stained with CD3 (17A2) PE and UC7-13D5 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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107512 100 µg $229
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro, and to deplete peripheral TCR-γ/δ-bearing T cells in vivo.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4. Although several papers have reported in vivo depletion3, 5,6, another study found that the effect of the antibody could be the functional blocking of the TCR signaling, rather than cell depletion7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107515 - 107520).

Though clone UC7-13D5 has been used for the depletion of TCR γ/δ T cells in vivo3-5, there is a report suggesting that binding of clone UC7-13D5 to the γ/δ TCR leads to downregulation of the γ/δ TCR, making the γ/δ T cells undetectable although they are still present7.

Application References

(PubMed link indicates BioLegend citation)
  1. Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. (Costim)
  3. Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. (Deplete)
  4. Dieli F, et al. 1997. Eur. J. Immunol. 27:206. (Costim)
  5. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  6. Pollinger B, et al. 2011. J. Immunol. 186:2602. PubMed
  7. Koenecke C, et al. 2009. Eur J Immunol. 39(2):372-9.
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  3. Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
  4. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
RRID
AB_492900 (BioLegend Cat. No. 107512)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex
Distribution

T subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum

Function
Antigen recognition
Ligand/Receptor
Some bacterial or tumor antigens bound MHC class I
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Skarstein K, et al. 1995. Immunology 81:497.
2. Harrison LC, et al. 1996. J. Exp. Med. 184:2167.
3. Wildner G, et al. 1996. Eur. J. Immunol. 26:2140.
4. Brandes M, et al. 2005. Science 309:264.

Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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