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Alexa Fluor® 594 anti-Alix Antibody

Pricing & Availability
Clone
3A9 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
PDCD6IP, PDCD6 interacting protein, AIP1, Hp95
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3A9_AF594_Alix_Antibody_ICC_042319
NIT/3T3 cells were fixed with -20°C Methanol for ten minutes and then blocked with 5% FBS for 30 minutes. The cells were stained with 5 µg/ml of anti-Alix (Clone 3A9) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 40X objective.
  • 3A9_AF594_Alix_Antibody_ICC_042319
    NIT/3T3 cells were fixed with -20°C Methanol for ten minutes and then blocked with 5% FBS for 30 minutes. The cells were stained with 5 µg/ml of anti-Alix (Clone 3A9) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 40X objective.
See Alexa Fluor® 594 spectral data
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634504 100 µg $382
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Description

Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. The predicted molecular weight is approximately 96 kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. The Bro1 domain interacts with CHMP4b, which is also involved in endosomal sorting.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
Rat, Frog
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full-length human Alix protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Clone 3A9 has been shown to be useful for Western blotting1, immunoprecipitation, and immunofluorescence2 staining of the human and mouse Alix protein.

Clone 3A9 shows strong specificity for mouse Alix.

Application References

(PubMed link indicates BioLegend citation)
  1. Morel L, et al. 2013. J Biol Chem. 288:7105. (WB) PubMed
  2. Pan S, et al. 2008. EMBO J. 27:2077. (IF)
  3. Ertunc ME, et al. 2015. J Lipid Res. 56:423. PubMed
Product Citations
  1. Salybekov AA, et al. 2021. Front Cardiovasc Med. 8:754254. PubMed
RRID
AB_2565615 (BioLegend Cat. No. 634504)

Antigen Details

Structure
Belongs to a conserved family of proteins that have in common an N-terminal Bro1 domain and a C-terminal proline-rich domain. Predicted molecular weight approximately 96 kD.
Distribution

Cytoplasm. Alix is also abundant within extracellular vesicles known as exosomes, and enriched in virions that are released from cells under the direction of the HIV-1 Gag protein.

Function
Adaptor in signal transduction pathways. Involved in apoptosis and endosomal membrane trafficking and viral budding.
Interaction
ALG2, SH3GL2, H-Vps28, CHMP4B, CHMP4A, CHMP4C, SETA, CIN85, Src
Biology Area
Cell Biology
Antigen References

1. Wu Y, et al. 2000. Differentiation 67:139.
2. Missotten M, et al. 1999. Cell Death Differ. 6:124.
3. Odorizzi G. 2006. J Cell Sci. 119:3025.

Gene ID
10015 View all products for this Gene ID
UniProt
View information about Alix on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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