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Alexa Fluor® 594 anti-human CD21 Antibody

Pricing & Availability
Clone
Bu32 (See other available formats)
Regulatory Status
RUO
Workshop
V CD21.4, VI CD21.5
Other Names
Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
Bu32_A594_CD21_Antibody_1_101023
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD21 (clone BU32) Alexa Fluor® 594 (red) at 4°C overnight. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • Bu32_A594_CD21_Antibody_1_101023
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD21 (clone BU32) Alexa Fluor® 594 (red) at 4°C overnight. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • Bu32_A594_CD21_Antibody_2_101023
    Human paraffin-embedded brain tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD21 (clone BU32) Alexa Fluor® 594 (red) at 4°C overnight. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
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354933 25 µg $175
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354934 100 µg $350
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Description

CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Björck P, et al. 1993. Eur. J. Immunol. 23:1771.
  2. Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497.
  3. Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369.
  4. Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC)
RRID
AB_3105989 (BioLegend Cat. No. 354933)
AB_3105989 (BioLegend Cat. No. 354934)

Antigen Details

Structure
Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD
Distribution

Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells

Function
Epstein-Barr virus receptor on B cells used for infection
Interaction
CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1
Ligand/Receptor
C3d, EBV
Cell Type
B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes
Biology Area
Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc.
2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194.
3. Szakonyi G, et al. 2001. Science 292:1725.
4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881.

Gene ID
1380 View all products for this Gene ID
UniProt
View information about CD21 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/11/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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